Petersson B A, Stålenheim G
Int Arch Allergy Appl Immunol. 1977;55(1-6):178-88. doi: 10.1159/000231925.
When serum was fractionated on Sephadex G-200, the material eluted in the second and thir major peaks had a very pronounced capacity to enhance IgE- as well as IgG-mediated histamine release from the leukocytes of both normal and allergic donors. Unseparated serum on the other hand had a low capacity to stimulate anti-IgE-induced histamine release. Besides resulting in a higher histamine release, pretreatment with serum fractions also increased the rate of histamine release. Further purification revealed that the stimulating activity of the material in the second peak was mediated by a trypsin-sensitive component, probably active in low concentration.
当血清在葡聚糖G - 200上进行分级分离时，在第二和第三个主要峰中洗脱出来的物质具有非常显著的能力，可增强来自正常和过敏供体白细胞的IgE以及IgG介导的组胺释放。另一方面，未分离的血清刺激抗IgE诱导的组胺释放的能力较低。除了导致更高的组胺释放外，用血清组分进行预处理还提高了组胺释放的速率。进一步纯化显示，第二个峰中物质的刺激活性由一种对胰蛋白酶敏感的成分介导，可能在低浓度下具有活性。
