Zinner S H, Husson M, Klastersky J
J Infect Dis. 1981 Dec;144(6):583-7. doi: 10.1093/infdis/144.6.583.
An in vitro model is described that simulates serum and tissue levels of antibiotic, allowing the exposure of bacteria to changing concentrations of antibiotic with little dilution of the inoculum. A bacterial suspension is placed in the outer chamber of a unit through which runs a bundle of 150 polysulfone hollow fibers that retain proteins of greater than 10,000 daltons. These "capillaries" are in contact with the tubular lumen of a perfusion system. Antibiotic is injected into the tubing and circulated by a pump. At desired intervals given volumes of perfusate are removed and replaced with antibiotic-free broth (resulting in decreasing drug concentrations), or a constant level can be maintained. Samples from the outer chamber are taken for determination of bacterial counts and antibiotic levels. This method allows for the in vitro determination of persistent antibiotic effects and comparison of bolus and continuous infusions as well as the effects of single or combined antibodies on bacterial killing and regrowth.
本文描述了一种体外模型，该模型可模拟抗生素的血清和组织水平，使细菌暴露于浓度不断变化的抗生素中，同时接种物的稀释度很小。将细菌悬液置于一个装置的外腔中，一束150根聚砜中空纤维穿过该外腔，这些纤维可保留分子量大于10000道尔顿的蛋白质。这些“毛细管”与灌注系统的管状内腔接触。抗生素注入管道并由泵循环。按所需间隔取出一定体积的灌注液，并用无抗生素肉汤替换（导致药物浓度降低），或者可以维持恒定水平。从外腔采集样本以测定细菌计数和抗生素水平。该方法可用于体外测定抗生素的持续作用，比较推注和持续输注，以及单一或联合抗体对细菌杀灭和再生长的影响。
