Exocrine secretion of canine pancreas perfused extracorporeally.

A canine gastroduodenopancreatic block was isolated and perfused extracorporeally with homologous blood. This preparation was used for studying stimulated exocrine secretion of the pancreas. Stimulants were infused intra-arterially. Both vagi were stimulated electrically. Hydrochloric acid or L-phenylalanine were instilled intragastrically. Pancreatic secretion induced by secretin and during HCl instillation was rich in bicarbonate and poor in total protein. Pancreatic secretion obtained during the infusion of normal saline, cholecystokinin, bethanechol chloride or acetylcholine chloride and during instillation of amino acid as well as during electrical stimulation of vagi, contained less bicarbonate and more protein than secretin-induced secretion.