Kowalewski K, Kolodej A
Surg Gynecol Obstet. 1978 Mar;146(3):375-8.
A canine gastroduodenopancreatic block was isolated and perfused ex vivo with fluorocarbon emulsion, oxygenated in vitro, and containing a simulated physiologic protein-free solution. Pancreatic and gastric secretions were collected. The pancreas was stimulated with secretin or cholecystokinin infused intra-arterially. The response of the pancreas to both hormones was essentially identical to that observed under in vivo conditions or during perfusion of the isolated pancreas with homologous blood. Secretin induced pancreatic secretion was rich in bicarbonate and poor in total protein. Cholecystokinin induced pancreatic secretion contained less bicarbonate and more protein than secretin induced secretion. Differences between secretory responses of the pancreas to these hormones were significant. Gastric secretion was alkaline and contained fluorocarbon emulsion. The results of this study demonstrated that, under the experimental conditions described, external secretory function of the isolated canine pancreas was preserved during perfusion of isolated organs with fluorocarbon emulsion.
分离出犬的胃十二指肠胰腺块，在体外用氟碳乳剂灌注，在体外进行氧合，并含有模拟的生理性无蛋白溶液。收集胰腺和胃的分泌物。通过动脉内注入促胰液素或胆囊收缩素刺激胰腺。胰腺对这两种激素的反应与在体内条件下或用同源血液灌注分离的胰腺时观察到的反应基本相同。促胰液素诱导的胰腺分泌富含碳酸氢盐而总蛋白含量低。胆囊收缩素诱导的胰腺分泌比促胰液素诱导的分泌含有更少的碳酸氢盐和更多的蛋白质。胰腺对这些激素的分泌反应之间的差异是显著的。胃分泌液呈碱性并含有氟碳乳剂。本研究结果表明，在所描述的实验条件下，在用氟碳乳剂灌注分离器官期间，分离的犬胰腺的外分泌功能得以保留。
