Handsfield H H, Sandström E G, Knapp J S, Perine P L, Whittington W L, Sayers D E, Holmes K K
N Engl J Med. 1982 Apr 22;306(16):950-4. doi: 10.1056/NEJM198204223061602.
Auxotyping and serogrouping by coagglutination were used to characterize penicillinase-producing Neisseria gonorrhoeae and penicillinase-negative isolates from the state of Washington, Shreveport (Louisiana), and the Far East. Fifty-four of 75 penicillinase-producing isolates (72 per cent) from Washington required proline for growth and were serogroup W-l (Pro-1), the predominant type of penicillinase-producing strains in the Philippines; none of 86 penicillinase-negative isolates from Washington was Pro-1 (P less than 0.0001). All 38 penicillinase-producing isolates from Shreveport required proline and were serogroup W-11 (Pro-11); five of 26 penicillinase-negative isolates (19 per cent) from Shreveport were also Pro-11 (P less than 0.0001) but had antigenic specificities within serogroup W-ll that distinguished them from the penicillinase-producing isolates. We conclude that the Washington and Shreveport outbreaks resulted from the spread of imported strains rather than transmission of penicillinase-encoding plasmids to indigenous gonococci. The Shreveport outbreak involved a single strain of penicillinase-producing N, gonorrhoeae and probably originated from a common source, whereas several types were involved in the multiple-source Washington outbreak, indicating repeated introduction of new strains.
采用凝聚试验进行菌型鉴定和血清群分类，以对来自华盛顿州、什里夫波特（路易斯安那州）和远东地区的产青霉素酶淋病奈瑟菌及非产青霉素酶分离株进行特征分析。来自华盛顿的75株产青霉素酶分离株中有54株（72%）生长需要脯氨酸，血清群为W-1（Pro-1），这是菲律宾产青霉素酶菌株的主要类型；来自华盛顿的86株非产青霉素酶分离株中无一为Pro-1（P<0.0001）。来自什里夫波特的38株产青霉素酶分离株均需要脯氨酸，血清群为W-11（Pro-11）；来自什里夫波特的26株非产青霉素酶分离株中有5株（19%）也是Pro-11（P<0.0001），但在血清群W-11内具有与产青霉素酶分离株不同的抗原特异性。我们得出结论，华盛顿和什里夫波特的疫情是由输入菌株的传播引起的，而非青霉素酶编码质粒向本地淋球菌的传播。什里夫波特的疫情涉及单一菌株的产青霉素酶淋病奈瑟菌，可能源于同一来源，而多源的华盛顿疫情涉及多种类型，表明新菌株多次引入。
