Ansink-Schipper M C, van Klingeren B, Huikeshoven M H, Woudstra R K, Dessens-Kroon M, van Wijngaarden L J
Br J Vener Dis. 1984 Jun;60(3):141-6. doi: 10.1136/sti.60.3.141.
We carried out auxanographic typing and plasmid identification on 1380 isolates of penicillinase producing Neisseria gonorrhoeae (PPNG) isolated in the Netherlands in 1982 and found four plasmid patterns and 24 auxotypes with noticeable local or regional variations. Among 756 strains harbouring the 3 X 2 megadalton (Mdal) resistance plasmid ("African" type), with or without the 24 Mdal transfer plasmid, 667 (88%) were non-requiring and inhibited by phenylalanine. This type was endemic in most of the cities or regions we studied. Twenty methionine requiring PPNG strains were found, all harbouring the 3.2 and 24 Mdal plasmid; virtually all of them were isolated or contracted in the region of Groningen. The predominant (443 (71%) auxotype among the 624 PPNG strains containing the 4.5 Mdal plasmid ("Asian" type) (with or without the 24 Mdal plasmid) was proline requiring. This auxotype (with the 4.5 Mdal and 24 Mdal plasmid) caused an outbreak in Amsterdam, and in Groningen replaced the local methionine requiring auxotype which had the 3.2 Mdal and 24 Mdal plasmids. Many auxotypes with the 4.5 Mdal plasmid, and requiring proline only, or proline and isoleucine, circulated in the Hague. Spread of imported strains by prostitution played an important part in the epidemiology of infection with PPNG strains.
我们对1982年在荷兰分离出的1380株产青霉素酶淋病奈瑟菌（PPNG）进行了生长谱分型和质粒鉴定，发现了四种质粒图谱和24种生长型，存在明显的局部或区域差异。在756株携带3×2兆道尔顿（Mdal）耐药质粒（“非洲”型）的菌株中，无论有无24 Mdal转移质粒，667株（88%）不需要苯丙氨酸且受其抑制。这种类型在我们研究的大多数城市或地区呈地方性流行。发现了20株需要甲硫氨酸的PPNG菌株，均携带3.2和24 Mdal质粒；实际上所有这些菌株都是在格罗宁根地区分离或感染的。在624株含有4.5 Mdal质粒（“亚洲”型）（无论有无24 Mdal质粒）的PPNG菌株中，占主导地位的生长型（443株（71%））需要脯氨酸。这种生长型（携带4.5 Mdal和24 Mdal质粒）在阿姆斯特丹引发了一次暴发，在格罗宁根取代了当地携带3.2 Mdal和24 Mdal质粒且需要甲硫氨酸的生长型。许多携带4.5 Mdal质粒且只需要脯氨酸或需要脯氨酸和异亮氨酸的生长型在海牙传播。通过卖淫传播输入性菌株在PPNG菌株感染的流行病学中起了重要作用。
