Lopez E, Tisserand-Jochem E M, Eyquem A, Milet C, Hillyard C, Lallier F, Vidal B, MacIntyre I
C R Seances Acad Sci III. 1981 Dec 7;293(13):707-12.
In eels the parathyrin of the corpuscles of Stannius (PCS), mammalian parathyroid-like hormone, has been localized in the cytoplasm of all the cells in the corpuscles. This detection was done by indirect immunofluorescence with an antiserum anti 1-84 bovine parathormone. The specificity of the reaction was demonstrated by inhibition of the coloration obtained with the 1-84 bovine parathormone and the active fragment 1-34 of human parathormone. Variations of the cellular localization of the PCS or a complete depletion of the hormonal content were observed in eels made hypercalcaemic by calcium overloading.
在鳗鱼中，斯坦尼小体（PCS）的类甲状旁腺素（与哺乳动物甲状旁腺激素类似）已定位在小体所有细胞的细胞质中。这一检测是通过使用抗1-84牛甲状旁腺激素抗血清进行间接免疫荧光法完成的。通过用1-84牛甲状旁腺激素和人甲状旁腺激素的活性片段1-34抑制所获得的显色反应，证明了该反应的特异性。在因钙超载而血钙过高的鳗鱼中，观察到了PCS细胞定位的变化或激素含量的完全耗尽。
