Kedar I, Sohar E, Ravid M
J Lab Clin Med. 1982 May;99(5):693-700.
ADA of human serum was demonstrated and investigated with an agar gel diffusion technique using amyloid-impregnated agar plates. Sera of 20 healthy adults, 40 patients with AA-amyloidosis, and 86 nonamyloidotic patients were tested. The presence of an ADF, showing enzymatic properties and strongly bound to albumin, was demonstrated in normals and amyloidotic and nonamyloidotic patients. ADA in the serum of amyloidotic and cirrhotic patients was markedly decreased due to the presence of an inhibitor of ADF. ADA of amyloidotic sera was restored to normal by EDTA, citric acid, and ascorbic acid. The ADA of 16 FMF patients and four of 34 patients with rheumatoid arthritis without amyloidosis was intermediate between normal and amyloidotic values, indicating the presence of lADF at low concentrations in these patients. These findings suggest that amyloid is a normal protein metabolite, possibly with a high metabolic turnover. Accumulation of amyloid may be caused by decrease of the ADA of the serum by its inhibitor, rather than by accelerated production.
采用淀粉样物质浸渍琼脂平板的琼脂凝胶扩散技术，对人血清中的ADA进行了演示与研究。测试了20名健康成年人、40名AA型淀粉样变性患者以及86名非淀粉样变性患者的血清。在正常人和淀粉样变性及非淀粉样变性患者中均证实存在一种显示酶活性且与白蛋白紧密结合的ADF。由于存在ADF抑制剂，淀粉样变性和肝硬化患者血清中的ADA显著降低。淀粉样变性血清中的ADA可通过EDTA、柠檬酸和抗坏血酸恢复正常。16名家族性地中海热（FMF）患者以及34名无淀粉样变性的类风湿关节炎患者中的4名患者的ADA介于正常和淀粉样变性值之间，表明这些患者中存在低浓度的lADF。这些发现表明，淀粉样物质是一种正常的蛋白质代谢产物，可能具有较高的代谢周转率。淀粉样物质的积累可能是由其抑制剂导致血清ADA降低引起的，而非由加速产生所致。
