Turner D P, Gregson N A
Parasitology. 1982 Apr;84(Pt 2):227-38. doi: 10.1017/s0031182000044796.
The cell surface properties of Plasmodium gallinaceum sporozoites have been investigated by means of microelectrophoretic and lectin-binding studies. Their electrophoretic mobility has been measured as a function of pH, the results suggesting qualitative and quantitative differences in the surface ionogenic groups between sporozoites from mature oocysts and those from salivary glands. Reaction of sporozoites with citraconic anhydride produced a small but significant increase in mobility, whereas 5,5-dithio-bis-(2-nitrobenzoic) acid had no effect on mobility; thus there appear to be amino groups but not -SH groups at the surface of sporozoites. Treatment of sporozoites with trypsin considerably reduced their mobility and suggests that a significant proportion of the cell surface charge is associated with protein. Incubation with neuraminidase, however, had no effect on sporozoite mobility and indicates that sialic acid residues, responsible for much of the negative charge associated with mammalian cells, are probably not present on the cell surface of sporozoites. Evidence for the presence of carbohydrates on the cell surface membrane of sporozoites was sought using fluorescein isothiocyanate-Concanavalin A. Results demonstrated that ligands similar to alpha-D-glucose and alpha-D-mannose are not present in an exposed or reactive form on the cell surface membrane of P. gallinaceum sporozoites.
通过微电泳和凝集素结合研究对鸡疟原虫子孢子的细胞表面特性进行了研究。测量了它们的电泳迁移率作为pH的函数，结果表明成熟卵囊的子孢子与唾液腺的子孢子之间表面离子ogenic基团在质和量上存在差异。子孢子与柠康酸酐反应导致迁移率有小幅但显著的增加，而5,5-二硫代双-(2-硝基苯甲酸)对迁移率没有影响；因此，子孢子表面似乎存在氨基但不存在-SH基团。用胰蛋白酶处理子孢子会大大降低它们的迁移率，这表明细胞表面电荷的很大一部分与蛋白质有关。然而，用神经氨酸酶孵育对子孢子迁移率没有影响，这表明负责与哺乳动物细胞相关的大部分负电荷的唾液酸残基可能不存在于子孢子的细胞表面。使用异硫氰酸荧光素-伴刀豆球蛋白A寻找子孢子细胞膜表面存在碳水化合物的证据。结果表明，类似于α-D-葡萄糖和α-D-甘露糖的配体在鸡疟原虫子孢子的细胞膜表面不是以暴露或反应性形式存在的。
