雌酮UDP-葡萄糖醛酸基转移酶和对硝基苯酚UDP-葡萄糖醛酸基转移酶的磷脂依赖性
Phospholipid-dependence of oestrone UDP-glucuronyltransferase and p-nitrophenol UDP-glucuronyltransferase.
作者信息
Tukey R H, Billings R E, Autor A P, Tephly T R
出版信息
Biochem J. 1979 Apr 1;179(1):59-65. doi: 10.1042/bj1790059.
Hepatic UDP-glucuronyltransferase activity was resolved into two fractions, one exhibiting oestrone glucuronyltransferase activity and the other exhibiting p-nitrophenol glucuronyltransferase activity. Hydroxyapatite-column chromatography removed greater than 95% of the phospholipids from both preparations. The partially purified delipidated enzymes were essentially devoid of catalytic activity, but activities were restored by the addition of phospholipids or phosphatidylcholine mixtures containing various saturated and unsaturated fatty acids. Both oestrone and p-nitrophenol glucuronyl-transferase activities were reconstituted to similar degrees with the phosphatidylcholine mixtures. When purified phospholipids were tested, phosphatidylcholine and lysophosphatidylcholine were most effective in restoring activity, whereas phosphatidylethanolamine was the least effective. These results further suggest that oestrone and p-nitrophenol UDP-glucuronyltransferases are dependent on phospholipids for their activity.
肝脏UDP-葡萄糖醛酸基转移酶活性被分离为两个部分，一个表现出雌酮葡萄糖醛酸基转移酶活性，另一个表现出对硝基苯酚葡萄糖醛酸基转移酶活性。羟基磷灰石柱色谱法从两种制剂中去除了超过95%的磷脂。部分纯化的脱脂酶基本上没有催化活性，但通过添加磷脂或含有各种饱和和不饱和脂肪酸的磷脂酰胆碱混合物可恢复活性。雌酮和对硝基苯酚葡萄糖醛酸基转移酶活性都能被磷脂酰胆碱混合物以相似程度重建。当测试纯化的磷脂时，磷脂酰胆碱和溶血磷脂酰胆碱在恢复活性方面最有效，而磷脂酰乙醇胺最无效。这些结果进一步表明，雌酮和对硝基苯酚UDP-葡萄糖醛酸基转移酶的活性依赖于磷脂。
Zotero 插件