• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

肺炎链球菌青霉素耐药临床分离株中青霉素结合蛋白的多种变化

Multiple changes of penicillin-binding proteins in penicillin-resistant clinical isolates of Streptococcus pneumoniae.

作者信息

Hakenbeck R, Tarpay M, Tomasz A

出版信息

Antimicrob Agents Chemother. 1980 Mar;17(3):364-71. doi: 10.1128/AAC.17.3.364.

DOI:10.1128/AAC.17.3.364
PMID:7425601
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283792/
Abstract

Penicillin-binding properties and characteristics of penicillin-binding proteins (PBPs) were investigated in several clinical isolates of Streptococcus pneumoniae differing in their susceptibilities to penicillin (minimal inhibitory concentration [MIC], 0.03 to 0.5 microgram/ml) and compared with the penicillin-susceptible strain R36A (MIC, 0.07 microgram/ml). Several changes accompanied the development of resistance: the relative affinity to penicillin of whole cells, isolated membranes, and two major PBPs after in vivo or in vitro labeling decreased (with increasing resistance). Furthermore, one additional PBP (2') appeared in four of five relatively resistant strains with an MIC of 0.25 microgram/ml and higher. PBP 3 maintained the same high affinity toward penicillin in all strains under all labeling conditions.

摘要

对几种对青霉素敏感性不同（最低抑菌浓度[MIC]为0.03至0.5微克/毫升）的肺炎链球菌临床分离株的青霉素结合特性及青霉素结合蛋白（PBPs）的特征进行了研究，并与青霉素敏感菌株R36A（MIC为0.07微克/毫升）进行了比较。耐药性的产生伴随着几个变化：体内或体外标记后，全细胞、分离膜和两种主要PBPs对青霉素的相对亲和力降低（耐药性增加）。此外，在五株MIC为0.25微克/毫升及更高的相对耐药菌株中，有四株出现了一种额外的PBP（2'）。在所有标记条件下，所有菌株中的PBP 3对青霉素都保持相同的高亲和力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e6f/283792/8210a4cde9f9/aac00383-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e6f/283792/f7153770f218/aac00383-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e6f/283792/8210a4cde9f9/aac00383-0083-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e6f/283792/f7153770f218/aac00383-0083-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6e6f/283792/8210a4cde9f9/aac00383-0083-b.jpg

相似文献

1
Multiple changes of penicillin-binding proteins in penicillin-resistant clinical isolates of Streptococcus pneumoniae.肺炎链球菌青霉素耐药临床分离株中青霉素结合蛋白的多种变化
Antimicrob Agents Chemother. 1980 Mar;17(3):364-71. doi: 10.1128/AAC.17.3.364.
2
Alterations in penicillin-binding proteins of clinical and laboratory isolates of pathogenic Streptococcus pneumoniae with low levels of penicillin resistance.青霉素耐药水平较低的致病性肺炎链球菌临床和实验室分离株中青霉素结合蛋白的改变
J Infect Dis. 1986 Jan;153(1):83-9. doi: 10.1093/infdis/153.1.83.
3
Penicillin-binding protein families: evidence for the clonal nature of penicillin resistance in clinical isolates of pneumococci.青霉素结合蛋白家族：肺炎球菌临床分离株中青霉素耐药性克隆性质的证据。
J Infect Dis. 1989 Jan;159(1):16-25. doi: 10.1093/infdis/159.1.16.
4
The alteration of penicillin-binding proteins (PBPs) in drug-resistant Streptococcus pneumoniae isolated from acute otitis media.从急性中耳炎分离出的耐药肺炎链球菌中青霉素结合蛋白（PBPs）的改变。
Acta Otolaryngol Suppl. 1999;540:67-71.
5
Interaction of beta-lactam antibiotics with the penicillin-binding proteins of penicillin-resistant Streptococcus pneumoniae.β-内酰胺类抗生素与耐青霉素肺炎链球菌青霉素结合蛋白的相互作用。
Chemotherapy. 1995 May-Jun;41(3):159-64. doi: 10.1159/000239338.
6
Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae.多重耐药的南非肺炎链球菌菌株的青霉素结合蛋白
Antimicrob Agents Chemother. 1980 Mar;17(3):434-42. doi: 10.1128/AAC.17.3.434.
7
[Evaluation of penicillin-binding protein genotypes in penicillin susceptible and resistant Streptococcus pneumoniae isolates].[青霉素敏感和耐药肺炎链球菌分离株中青霉素结合蛋白基因型的评估]
Mikrobiyol Bul. 2012 Apr;46(2):190-201.
8
Effects of amino acid alterations in penicillin-binding proteins (PBPs) 1a, 2b, and 2x on PBP affinities of penicillin, ampicillin, amoxicillin, cefditoren, cefuroxime, cefprozil, and cefaclor in 18 clinical isolates of penicillin-susceptible, -intermediate, and -resistant pneumococci.青霉素结合蛋白（PBPs）1a、2b和2x中氨基酸改变对18株青霉素敏感、中介及耐药肺炎链球菌临床分离株中青霉素、氨苄西林、阿莫西林、头孢妥仑、头孢呋辛、头孢丙烯和头孢克洛的PBP亲和力的影响
Antimicrob Agents Chemother. 2002 May;46(5):1273-80. doi: 10.1128/AAC.46.5.1273-1280.2002.
9
Penicillin-binding proteins of Streptococcus pneumoniae.肺炎链球菌的青霉素结合蛋白
J Antimicrob Chemother. 1988 Dec;22(6):791-800. doi: 10.1093/jac/22.6.791.
10
Association of a thr-371 substitution in a conserved amino acid motif of penicillin-binding protein 1A with penicillin resistance of Streptococcus pneumoniae.肺炎链球菌青霉素结合蛋白1A保守氨基酸基序中的苏氨酸-371替代与青霉素耐药性的关联
Antimicrob Agents Chemother. 1998 Sep;42(9):2267-73. doi: 10.1128/AAC.42.9.2267.

引用本文的文献

1
Differential gene expression analysis shows that cephalosporin resistance is intrinsic to Clostridioides difficile strain 630.差异基因表达分析表明，艰难梭菌630菌株对头孢菌素的耐药性是内在的。
J Antibiot (Tokyo). 2025 Jan;78(2):113-125. doi: 10.1038/s41429-024-00795-3. Epub 2024 Dec 13.
2
Biological puzzles solved by using : a historical review of the pneumococcal studies that have impacted medicine and shaped molecular bacteriology.运用历史回顾的方法解决生物学难题：对影响医学和分子细菌学发展的肺炎球菌研究的回顾。
J Bacteriol. 2024 Jun 20;206(6):e0005924. doi: 10.1128/jb.00059-24. Epub 2024 May 29.
3
Invasive Group A Streptococcal Penicillin Binding Protein 2× Variants Associated with Reduced Susceptibility to β-Lactam Antibiotics in the United States, 2015-2021.

本文引用的文献

1
The site of action of penicillin. 1. Uptake of penicillin on bacteria.青霉素的作用位点。1. 青霉素在细菌上的摄取。
Biochem J. 1950 Feb;46(2):157-61. doi: 10.1042/bj0460157.
2
PENICILLIN UPTAKE BY BACTERIAL CELLS.细菌细胞对青霉素的摄取
J Bacteriol. 1949 Apr;57(4):415-22. doi: 10.1128/jb.57.4.415-422.1949.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
美国 2015-2021 年与β-内酰胺类抗生素耐药性降低相关的侵袭性 A 组链球菌青霉素结合蛋白 2x 变体。
Antimicrob Agents Chemother. 2022 Sep 20;66(9):e0080222. doi: 10.1128/aac.00802-22. Epub 2022 Aug 15.
4
Emergence of Ceftriaxone Resistance during a Case of Pneumococcal Meningitis with Fatal Evolution.在一例具有致命性演变的肺炎球菌性脑膜炎病例中出现头孢曲松耐药性。
Antimicrob Agents Chemother. 2020 Feb 21;64(3). doi: 10.1128/AAC.01958-19.
5
Puzzling Over the Pneumococcal Pangenome.对肺炎球菌泛基因组的困惑
Front Microbiol. 2018 Oct 30;9:2580. doi: 10.3389/fmicb.2018.02580. eCollection 2018.
6
Biological and Epidemiological Features of Antibiotic-Resistant Streptococcus pneumoniae in Pre- and Post-Conjugate Vaccine Eras: a United States Perspective.结合疫苗时代前后美国耐抗生素肺炎链球菌的生物学和流行病学特征
Clin Microbiol Rev. 2016 Jul;29(3):525-52. doi: 10.1128/CMR.00058-15.
7
Profiling of β-lactam selectivity for penicillin-binding proteins in Streptococcus pneumoniae D39.肺炎链球菌D39中青霉素结合蛋白的β-内酰胺选择性分析
Antimicrob Agents Chemother. 2015;59(6):3548-55. doi: 10.1128/AAC.05142-14. Epub 2015 Apr 6.
8
Population genomics of post-vaccine changes in pneumococcal epidemiology.疫苗接种后肺炎链球菌流行病学变化的群体基因组学研究。
Nat Genet. 2013 Jun;45(6):656-63. doi: 10.1038/ng.2625. Epub 2013 May 5.
9
The 2011 Garrod Lecture: From penicillin-binding proteins to molecular epidemiology.2011年加罗德讲座：从青霉素结合蛋白到分子流行病学
J Antimicrob Chemother. 2012 Jul;67(7):1578-88. doi: 10.1093/jac/dks109. Epub 2012 Mar 28.
10
In vitro evaluation of the antimicrobial activity of ceftaroline against cephalosporin-resistant isolates of Streptococcus pneumoniae.头孢洛林对耐头孢菌素肺炎链球菌分离株抗菌活性的体外评价
Antimicrob Agents Chemother. 2009 Feb;53(2):552-6. doi: 10.1128/AAC.01324-08. Epub 2008 Nov 17.
J Biol Chem. 1951 Nov;193(1):265-75.
4
The binding of penicillin in relation to its cytotoxic action. II. The reactivity with penicillin of resistant variants of streptococci, pneumococci, and staphylococci.青霉素结合与其细胞毒性作用的关系。II. 链球菌、肺炎球菌和葡萄球菌耐药变体与青霉素的反应性。
J Exp Med. 1954 Jul 1;100(1):103-15. doi: 10.1084/jem.100.1.103.
5
The binding of penicillin in relation to its cytotoxic action. I. Correlation between the penicillin sensitivity and combining activity of intact bacteria and cell-free extracts.青霉素结合与其细胞毒性作用的关系。I. 完整细菌和无细胞提取物的青霉素敏感性与结合活性之间的相关性。
J Exp Med. 1954 Mar;99(3):207-26. doi: 10.1084/jem.99.3.207.
6
Penicillin-binding proteins of multiply antibiotic-resistant South African strains of Streptococcus pneumoniae.多重耐药的南非肺炎链球菌菌株的青霉素结合蛋白
Antimicrob Agents Chemother. 1980 Mar;17(3):434-42. doi: 10.1128/AAC.17.3.434.
7
Correlation between growth inhibition and the binding of various penicillins and cephalosporins to Staphylococcus aureus.生长抑制与各种青霉素和头孢菌素与金黄色葡萄球菌结合之间的相关性。
J Bacteriol. 1969 Aug;99(2):459-62. doi: 10.1128/jb.99.2.459-462.1969.
8
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
9
Increased resistance to penicillin of pneumococci isolated from man.从人类分离出的肺炎球菌对青霉素的耐药性增强。
N Engl J Med. 1971 Jan 28;284(4):175-7. doi: 10.1056/NEJM197101282840403.
10
Cellular metabolism in genetic transformation of pneumococci: requirement for protein synthesis during induction of competence.肺炎链球菌遗传转化中的细胞代谢：感受态诱导过程中蛋白质合成的需求
J Bacteriol. 1970 Mar;101(3):860-71. doi: 10.1128/jb.101.3.860-871.1970.