白细胞介素I的缓冲液等电聚焦
Buffer electrofocusing of Interleukin I.
作者信息
Prestidge R L, Koopman W J, Bennett J C, Hearn M T
出版信息
Biosci Rep. 1982 Apr;2(4):241-6. doi: 10.1007/BF01136723.
PMID:6803852
Abstract
Buffer electrofocusing, utilizing a mixture of low-molecular-weight buffer compounds to establish a stable linear pH gradient covering the range 3.5-6.0 on granulated gel media, has been employed in the purification of Interleukin I with good recovery of biological activity. The difficulties often experienced in the removal of commercial synthetic ampholytes after conventional preparative electrofocusing are avoided in this method.
摘要
缓冲液等电聚焦法利用低分子量缓冲化合物的混合物在颗粒状凝胶介质上建立覆盖3.5至6.0范围的稳定线性pH梯度，已被用于白细胞介素I的纯化，且生物活性回收率良好。该方法避免了传统制备性等电聚焦后去除市售合成两性电解质时经常遇到的困难。
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