等电聚焦中聚焦载体两性电解质的摩尔浓度和离子强度。

Molarity and ionic strength of focused carrier ampholytes in isoelectric focusing.

作者信息

Righetti P G

出版信息

J Chromatogr. 1980 Apr 4;190(2):275-82. doi: 10.1016/s0021-9673(00)88230-8.

Abstract

Focused 1% carrier ampholytes in the pH range 3.5--10 have a molarity of 9--10 mM, as determined by ismolarity measurements of fractions focused in free water. From electrophoretic and isoelectric focusing data of red blood cells, it has been demonstrated that the corresponding ionic strength is 0.5 mg-ions/l. Also, theoretical considerations and conductivity measurements point to a value in the range of 0.5--1.0 mg-ions/l. The following equations for ionic strength (I) calculations have been derived: 1 = 1/20 C amph + CH in the pH range 2.5--7 and I = 1/20 Camph + COH in the pH range 7--11.

摘要

通过对聚焦于游离水中的组分进行等摩尔测量确定，pH范围为3.5 - 10的1%载体两性电解质的摩尔浓度为9 - 10 mM。根据红细胞的电泳和等电聚焦数据表明，相应的离子强度为0.5毫克离子/升。此外，理论考量和电导率测量结果表明该值在0.5 - 1.0毫克离子/升范围内。已推导出以下用于计算离子强度（I）的方程：在pH范围2.5 - 7时，I = 1/20 C两性 + CH；在pH范围7 - 11时，I = 1/20 C两性 + COH 。