关于紧密连接结构。

On tight-junction structure.

作者信息

Pinto da Silva P, Kachar B

出版信息

Cell. 1982 Mar;28(3):441-50. doi: 10.1016/0092-8674(82)90198-2.

Abstract

We have analyzed previous thin-section and freeze-fracture observations of the tight junction. We propose that the tight-junction strands represent intramembranous, cylindrical, inverted micelles. At the junctional site, the exoplasmic halves of the plasma membranes are fused into a continuous leaflet. Therefore, topologically and structurally the tight junction is viewed as the outcome of a process of linear fusion between the plasma membranes of epithelial cells. The extracellular spaces delimited by the junction are separated by two distinct exoplasmic membrane halves and the cylindrical micelles. Junctional stability, fostered by the environmental symmetry of the cytoplasmic milieux of contiguous cells, may be maintained by transmembrane integral proteins at the junctional site, interacting at the cytoplasmic surface with cytoskeletal components.

摘要

我们分析了先前对紧密连接的超薄切片和冷冻断裂观察结果。我们提出，紧密连接链代表膜内的圆柱形反向胶束。在连接部位，质膜的细胞外半部分融合成一个连续的薄片。因此，从拓扑结构上看，紧密连接被视为上皮细胞质膜之间线性融合过程的结果。由连接界定的细胞外空间被两个不同的细胞外膜半部分和圆柱形胶束分隔开。相邻细胞质环境的对称性促进了连接的稳定性，可能由连接部位的跨膜整合蛋白维持，这些蛋白在细胞质表面与细胞骨架成分相互作用。