McClean S W, Ruddel M E, Gross E G, DeGiovanna J J, Peck G L
Clin Chem. 1982 Apr;28(4 Pt 1):693-6.
A "high-performance" liquid-chromatographic separation of retinoids (retinol, isotretinoin, all-trans retinoic acid, retinal, etretinate, and retinyl acetate) in serum is described. The separation was used in developing a quantitative assay for retinol (vitamin A) and two therapeutic analogs, isotretinoin (13-cis-retinoic acid) and etretinate (Ro 10-9359). The procedure requires 1 mL of serum. Overall analytical recovery for retinol, isotretinoin, and etretinate from serum was 100% (SD 7%). The between-day coefficient of variation for specimens with concentrations ranging from 0.70 to 0.95 mg/L was less than 4%. Normal reference intervals for serum retinol in men and women are 0.61 to 1.33 and 0.44 to 1.19 mg/L, respectively.
本文描述了血清中类视黄醇（视黄醇、异维A酸、全反式维甲酸、视黄醛、阿维A酯和醋酸视黄酯）的“高效”液相色谱分离方法。该分离方法用于开发视黄醇（维生素A）以及两种治疗性类似物异维A酸（13-顺式维甲酸）和阿维A酯（Ro 10-9359）的定量分析方法。该方法需要1毫升血清。血清中视黄醇、异维A酸和阿维A酯的总分析回收率为100%（标准差7%）。浓度范围为0.70至0.95毫克/升的样本日间变异系数小于4%。男性和女性血清视黄醇的正常参考区间分别为0.61至1.33毫克/升和0.44至1.19毫克/升。
