Murphy R F
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8523-6. doi: 10.1073/pnas.82.24.8523.
The existence of three distinct classes of endocytic vesicles that are part of the pathway of fluid-phase endocytosis has been demonstrated by flow cytometry. Amounts of fluorescent and scattered light were measured on a particle-by-particle basis for unfractionated whole cell lysates from cells incubated with fluorescein isothiocyanate-dextran. After 20 min two different fluorescent populations were observed, and after a 180-min incubation a third highly fluorescent population was found. Since the fluorescein isothiocyanate-dextran per fluid-phase vesicle should be a function solely of the external fluorescein isothiocyanate-dextran concentration, the existence of endocytic compartments with widely different amounts of fluorescence could result from a wide range of sizes of initial endocytic vesicles. However, the kinetics of appearance of the intermediate and highly fluorescent vesicles suggest that these compartments become labeled through fusion with the smaller primary endocytic vesicles.
流式细胞术已证实，液相内吞途径中存在三类不同的内吞囊泡。对于用异硫氰酸荧光素 - 葡聚糖孵育的细胞的未分级全细胞裂解物，逐个粒子测量荧光量和散射光量。孵育20分钟后，观察到两个不同的荧光群体，孵育180分钟后，发现了第三个高荧光群体。由于每个液相囊泡中的异硫氰酸荧光素 - 葡聚糖应该仅仅是外部异硫氰酸荧光素 - 葡聚糖浓度的函数，荧光量差异很大的内吞区室的存在可能是由于初始内吞囊泡大小范围很广。然而，中等荧光和高荧光囊泡出现的动力学表明，这些区室是通过与较小的初级内吞囊泡融合而被标记的。
