Sugio T, Kudo S, Tano T, Imai K
J Bacteriol. 1982 Jun;150(3):1109-14. doi: 10.1128/jb.150.3.1109-1114.1982.
Properties of a heat-labile glucose transport system in Thiobacillus ferrooxidans strain AP-44 were investigated with iron-grown cells. [14C]glucose was incorporated into cell fractions, and the cells metabolized [14C]glucose to 14CO2. Amytal, rotenone, cyanide, azide, 2,4-dinitrophenol, and dicyclohexylcarbodiimide strongly inhibited [14C]glucose uptake activity, suggesting the presence of an energy-dependent glucose transport system in T. ferrooxidans. Heavy metals, such as mercury, silver, uranium, and molybdate, markedly inhibited the transport activity at 1 mM. When grown on mixotrophic medium, the bacteria preferentially utilized ferrous iron as an energy source. When iron was exhausted, the cells used glucose if the concentration of ferrous sulfate in the medium was higher than 3% (wt/vol). However, when ferrous sulfate was lower than 1%, both of the energy sources were consumed simultaneously.
利用铁培养的细胞研究了氧化亚铁硫杆菌AP-44菌株中一种热不稳定葡萄糖转运系统的特性。[14C]葡萄糖被掺入细胞组分中，并且细胞将[14C]葡萄糖代谢为14CO2。阿米妥、鱼藤酮、氰化物、叠氮化物、2,4-二硝基苯酚和二环己基碳二亚胺强烈抑制[14C]葡萄糖摄取活性，这表明氧化亚铁硫杆菌中存在能量依赖性葡萄糖转运系统。重金属，如汞、银、铀和钼酸盐，在1 mM时显著抑制转运活性。当在混合营养培养基上生长时，细菌优先利用亚铁作为能量来源。当铁耗尽时，如果培养基中亚铁硫酸盐的浓度高于3%（重量/体积），细胞会利用葡萄糖。然而，当亚铁硫酸盐低于1%时，两种能量来源会同时被消耗。
