Hazani A, Silvian I, Tatarsky I, Spira G
Am J Med Sci. 1982 May-Jun;283(3):169-73. doi: 10.1097/00000441-198205000-00010.
An asymptomatic hypercalcemic IgG-Kappa myeloma patient is presented. A calcium-IgG complex was isolated and purified from the serum by column chromatography. Following further purification on a Sepharose protein A column, the IgG-Kappa molecule did not contain any detectable amount of calcium, indicating that calcium is loosely bound to IgG. The monoclonal IgG demonstrated a strong calcium binding capacity in vitro. The calcium-IgG complex in the serum was shown not to be saturated and thus could absorb more ionized calcium. In view of the presence of an abnormal monoclonal IgG capable of binding excess calcium, and the fact that the asymptomatic patient did not respond to the antihypercalcemic drugs, the treatment was stopped.
报告了一名无症状高钙血症IgG-κ型骨髓瘤患者。通过柱色谱法从血清中分离并纯化出钙-IgG复合物。在蛋白A琼脂糖柱上进一步纯化后，IgG-κ分子未检测到任何钙含量，表明钙与IgG结合不紧密。单克隆IgG在体外表现出很强的钙结合能力。血清中的钙-IgG复合物未饱和，因此可以吸收更多的离子钙。鉴于存在能够结合过量钙的异常单克隆IgG，且无症状患者对抗高钙血症药物无反应，故停止治疗。
