Vasseur F, Fontaine F, Strozyk F, Deminatti M M
C R Seances Acad Sci III. 1982 Jan 25;294(4):223-6.
Unfixed, isolated metaphase chromosomes were used as template in an in vitro RNA synthesis assay. In these conditions, no RNA synthesis was observed by autoradiography. Transcription was effective after treatment with methanol-acetic acid, HCl 0,2 N, NaCl 0,35 M or pancreatic RNase. Transcription is the most important after treatment with RNase. This result points out the problem of the role of RNA in inhibition of chromosome during mitosis.
未固定的、分离的中期染色体在体外RNA合成试验中用作模板。在这些条件下，通过放射自显影未观察到RNA合成。用甲醇-乙酸、0.2N盐酸、0.35M氯化钠或胰核糖核酸酶处理后转录有效。用核糖核酸酶处理后转录最为有效。这一结果指出了RNA在有丝分裂期间对染色体抑制作用中的作用问题。
