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[中期染色体：与单链DNA序列存在相关的功能惰性和能力]

[The metaphase chromosome: functional inertia and ability related to the presence of sequences of single-stranded DNA].

作者信息

Vasseur F, Fontaine F, Savary J B, Deminatti M

出版信息

C R Acad Sci III. 1986;302(14):537-42.

PMID:3085880
Abstract

Isolated metaphase chromosomes from KB cells were used as template in an in vitro DNA synthesis assay. In these conditions, no synthesis was noticed, confirming template inactivity of isolated metaphase chromosomes. DNA synthesis was noticed after a pretreatment with either methanol-acetic acid or RNase A. Analysis of in vitro synthesized polydeoxyribonucleotides showed two fractions of 4 S and 7-8 S. These results suggest the presence in metaphase chromosome of single stranded DNA sequences. Such sequences are shown in DNA extracted from chromosomes. They would preexist in this organelle and would be unmasked by the treatments that restore template activity of metaphase chromosome.

摘要

在体外DNA合成试验中，将从KB细胞中分离得到的中期染色体用作模板。在这些条件下，未观察到合成现象，这证实了分离的中期染色体的模板无活性。在用甲醇-乙酸或核糖核酸酶A进行预处理后，观察到了DNA合成。对体外合成的多脱氧核糖核苷酸的分析显示出4S和7 - 8S两个组分。这些结果表明中期染色体中存在单链DNA序列。此类序列在从染色体中提取的DNA中可见。它们可能预先存在于该细胞器中，并会因恢复中期染色体模板活性的处理而暴露出来。

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1
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