Huber B T
Immunol Rev. 1982;64:57-79. doi: 10.1111/j.1600-065x.1982.tb00418.x.
In this review I have discussed the serological, biochemical and functional characterization of two differentiation antigens, Lyb3 and Ia. W39, which have the same time distribution; namely, they are selectively expressed on a late maturing subset of B cells (Lyb3 and Ia. W39) and antigen presenting macrophages (Ia. W39, Lyb3?) Antisera against both determinants were raised in xid defective F1 male mice, which were immunized with spleen cells from the normal parent. Lyb3 is an isogenic specificity expressed without allelic forms in all mouse strains, whereas Ia.W39 is a private specificity, encoded by a gene(s) within the I-Ab subregion of the H-2 complex. Interestingly, the xid gene does not control the synthesis of these differentiation antigens, but affects their membrane expression (shown for Ia. W39.) Lyb3 is a polypeptide of 68,000d MW which has a similar IE point in all mouse strains. The molecule bearing Ia. W39 has an identical 2-chain structure (a and beta) and 2-D gel profile as the molecule expressing all the conventional Ia specificities encoded by the I-Ab subregion. However, from the difference in the ontological appearance and the turnover rate and from sequential immunoprecipitation studies we concluded that there are two kinds of glycoproteins containing Aa and Abeta chains; both would express the conventional specificities, and one would, in addition, bear Ia. W39. Functionally, we have defined Lyb3 as a receptor for triggering signals and Ia. W39 as a specific Ir gene epitope.
在本综述中，我讨论了两种分化抗原Lyb3和Ia.W39的血清学、生化及功能特性，它们具有相同的时间分布；也就是说，它们在B细胞的一个晚期成熟亚群（Lyb3和Ia.W39）以及抗原呈递巨噬细胞（Ia.W39、Lyb3？）上选择性表达。针对这两种决定簇的抗血清是在xid缺陷的F1雄性小鼠中制备的，这些小鼠用正常亲代的脾细胞进行免疫。Lyb3是一种在所有小鼠品系中均以无等位基因形式表达的同基因特异性，而Ia.W39是一种私有特异性，由H-2复合体I-Ab亚区内的一个基因编码。有趣的是，xid基因并不控制这些分化抗原的合成，但会影响它们的膜表达（以Ia.W39为例）。Lyb3是一种分子量为68,000d的多肽，在所有小鼠品系中具有相似的IE点。携带Ia.W39的分子具有与表达由I-Ab亚区编码的所有传统Ia特异性的分子相同的2链结构（α和β）和二维凝胶图谱。然而，从本体出现和周转率的差异以及连续免疫沉淀研究中我们得出结论，存在两种含有αα和αβ链的糖蛋白；两者都将表达传统特异性，并且其中一种还将携带Ia.W39。在功能上，我们将Lyb3定义为触发信号的受体，将Ia.W39定义为特定的Ir基因表位。
