Andrew E M, Mackenzie N M, Parkhouse R M
Immunology. 1985 Feb;54(2):233-40.
Murine splenic B cells were stained with antibodies against mIg, Ia or FcR and then separated on the fluorescence-activated cell sorter on the basis of quantitative differences in marker expression; that is, they were fractionated into subpopulations bearing high or low densities of the marker. The separated cells were then tested for their relative capacities for T-dependent primary and secondary antibody responses, and for lipopolysaccharide responsiveness. There was no association between the surface density of any of these markers and the ability of the cells to proliferate in response to lipopolysaccharide. However, a high level of surface Ia was associated with good primary and secondary T-dependent responses. The density of mIg or of FcR showed no association with the capacity for primary responses, but a low density of these two markers, especially FcR, was correlated with good secondary responsiveness. Thus, subpopulations of B cells selected on the basis of quantitative levels of membrane markers can also be distinguished by their functional properties.
用抗mIg、Ia或FcR抗体对小鼠脾脏B细胞进行染色，然后根据标记物表达的定量差异在荧光激活细胞分选仪上进行分离；也就是说，它们被分成具有高或低密度标记物的亚群。然后测试分离的细胞对T细胞依赖性初次和二次抗体反应以及对脂多糖反应性的相对能力。这些标记物中任何一种的表面密度与细胞对脂多糖反应而增殖的能力之间均无关联。然而，高水平的表面Ia与良好的初次和二次T细胞依赖性反应相关。mIg或FcR的密度与初次反应能力无关，但这两种标记物的低密度，尤其是FcR的低密度，与良好的二次反应性相关。因此，根据膜标记物定量水平选择的B细胞亚群也可以通过其功能特性来区分。
