Rosenberger R F
Biochim Biophys Acta. 1976 Apr 23;428(2):516-24. doi: 10.1016/0304-4165(76)90060-x.
Phosphate starvation induced teichuronic acid synthesis in cells of Bacillus subtilis 168trp-which had previously been grown with excess phosphate. This induction was prevented when protein systhesis was inhibited immediately prior to phosphate starvation and under these conditions cells continued to form teichoic acid. The converse was true when phosphate was added to cells previously grown in a phosphate-limited chemostat. The increase in teichoic acid synthesis normally following phosphate addition was prevented by chloramphenicol or amino acid starvation and cells continued to make teichuronic acid. This suggestion that repression of enzyme synthesis is involved in controlling the type of wall polymer made was supported by the low levels of UDP-glucose dehydrogenase found in cells grown with excess phosphate and of CDP-glycerol pyrophosphorylase in phosphate-limited cells. The greater amounts of teichoic acid made under phosphate limitation and of teichuronic acid with excess phosphate when protein synthesis was also inhibited indicated that modulation of enzyme activity occurs. Glycerol starvation of a glycerol-requiring mutant did not derepress teichuronic acid synthesis, indicating that glycerol-containing imtermediates do not act as repressors.
磷酸盐饥饿诱导枯草芽孢杆菌168trp -细胞中磷壁酸质的合成，该菌株此前在过量磷酸盐条件下生长。当在磷酸盐饥饿之前立即抑制蛋白质合成时，这种诱导作用被阻止，在这些条件下细胞继续形成磷壁酸。当向先前在磷酸盐限制的恒化器中生长的细胞中添加磷酸盐时，情况则相反。通常在添加磷酸盐后磷壁酸合成的增加被氯霉素或氨基酸饥饿所阻止，细胞继续合成磷壁酸质。在过量磷酸盐条件下生长的细胞中发现的低水平UDP -葡萄糖脱氢酶以及在磷酸盐限制的细胞中发现的CDP -甘油焦磷酸化酶水平，支持了酶合成的抑制参与控制细胞壁聚合物类型的这一观点。当蛋白质合成也受到抑制时，在磷酸盐限制条件下产生的大量磷壁酸以及在过量磷酸盐条件下产生的大量磷壁酸质表明发生了酶活性的调节。对需要甘油的突变体进行甘油饥饿处理并没有解除对磷壁酸质合成的抑制，这表明含甘油的中间体不起阻遏物的作用。
