Mendelson N H, Keener S L
J Bacteriol. 1982 Jul;151(1):455-7. doi: 10.1128/jb.151.1.455-457.1982.
Helical macrofiber-producing strains of Bacillus subtilis grown on fresh complex medium semisolid surfaces formed "pinwheel"-shaped colonies. Clockwise pinwheel projections arose from colonies of strains that produce right-handed helical macrofibers in fluid cultures. Most strains able to make left-handed helical macrofibers in fluid grew as disorganized wavy colonies without directed projections. A phage-resistant left-handed mutant was found that produces very tight colonies with pinwheel projections that lie counterclockwise relative to the colony. The pinwheel colony morphology is interpreted therefore in terms of the cell surface organization and helical growth.
在新鲜复合培养基半固体表面生长的产螺旋状大纤维的枯草芽孢杆菌菌株形成了“风车”状菌落。顺时针方向的风车状突起源自那些在液体培养中产生右旋螺旋状大纤维的菌株的菌落。大多数在液体中能够产生左旋螺旋状大纤维的菌株生长为无组织的波浪状菌落，没有定向突起。发现了一种抗噬菌体的左旋突变体，它产生非常紧密的菌落，其风车状突起相对于菌落呈逆时针方向。因此，根据细胞表面组织和螺旋生长来解释风车状菌落形态。
