Le P T, Muller M T, Mortensen R F
J Immunol. 1982 Aug;129(2):665-72.
The acute phase reactant of mice, serum amyloid P-component (SAP), was purified and separated from C-reactive protein (CRP). The purified SAP is composed of identical M, 31 Kd polypeptide subunits, determined by SDS-PAGE. SAP levels increased five-fold by 24 hr after challenge with lipopolysaccharide (LPS) or thioglycollate. This response closely correlated with blood monocytosis and required new macromolecule (protein + RNA) synthesis and secretion by the liver. The induction of the SAP response was adoptively transferred by a serum factor produced in optimal concentrations only 90 min after an inflammatory stimulus, which preceded a detectable increase in SAP. A potent SAP inducer was identified in culture supernatants of LPS-activated macrophages. The rapid induction of SAP synthesis in LPS-unresponsive C3H/HeJ mice was dependent on the amount of lymphocyte-activating factor, LAF(IL 1), present in the macrophage culture supernatants. Partially purified human IL 1 also induced a rapid increase in SAP. Thus the induction of SAP in mice appears to be mediated by a product of macrophages, a cell population that is also expanded as part of the systemic inflammatory response.
从小鼠体内纯化并分离出急性期反应物血清淀粉样蛋白P成分（SAP）与C反应蛋白（CRP）。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）测定，纯化后的SAP由分子量均为31千道尔顿的相同多肽亚基组成。用脂多糖（LPS）或巯基乙酸盐攻击小鼠24小时后，SAP水平增加了五倍。这种反应与血液单核细胞增多密切相关，并且需要肝脏合成并分泌新的大分子（蛋白质+RNA）。炎症刺激后仅90分钟，血清中就会产生最佳浓度的血清因子，该因子可引发SAP反应，且发生在SAP可检测到的增加之前。在LPS激活的巨噬细胞培养上清液中鉴定出一种有效的SAP诱导剂。LPS无反应的C3H/HeJ小鼠中SAP合成的快速诱导取决于巨噬细胞培养上清液中淋巴细胞激活因子LAF（白细胞介素1）的含量。部分纯化的人白细胞介素1也能使SAP快速增加。因此，小鼠体内SAP的诱导似乎是由巨噬细胞的产物介导的，巨噬细胞群体作为全身炎症反应的一部分也会增多。
