Caterson B, Baker J R, Christner J E, Couchman J R
J Invest Dermatol. 1982 Jul;79 Suppl 1:45s-50s. doi: 10.1111/1523-1747.ep12545740.
In this paper we report the use of immunological methods for specifically detecting and determining proteoglycan in cartilage and other connective tissues. Antibodies (polyclonal and monoclonal) have been raised against specific components of cartilage proteoglycan aggregates (i.e., proteoglycan monomer and link protein). Radioimmunoassay procedures and immunohistochemical procedures have been developed and used to demonstrate the occurrence of cartilage-like proteoglycan and link protein in bovine aorta. Similarly, immunofluorescent studies have been used to analyze proteoglycan distribution in skin. Using antibodies specific for chondroitin-4-sulfated proteoglycan, their presence was demonstrated in dermal connective tissue and connective tissue surrounding nerve and muscle sheaths. However, chondroitin-4-sulfated proteoglycan was completely absent in the epidermis of skin and areas surrounding invaginating hair follicles. These immunological procedures are currently being used to complement conventional biochemical analyses of proteoglycans found in different connective tissue matrices.
在本文中，我们报告了利用免疫学方法特异性检测和测定软骨及其他结缔组织中蛋白聚糖的情况。已经制备了针对软骨蛋白聚糖聚集体特定成分（即蛋白聚糖单体和连接蛋白）的抗体（多克隆抗体和单克隆抗体）。已开发出放射免疫测定程序和免疫组织化学程序，并用于证明牛主动脉中存在软骨样蛋白聚糖和连接蛋白。同样，免疫荧光研究已用于分析皮肤中蛋白聚糖的分布。使用对硫酸软骨素-4-硫酸化蛋白聚糖具有特异性的抗体，在真皮结缔组织以及神经和肌肉鞘周围的结缔组织中证实了它们的存在。然而，硫酸软骨素-4-硫酸化蛋白聚糖在皮肤表皮和内陷毛囊周围区域完全不存在。这些免疫学程序目前正用于补充对不同结缔组织基质中发现的蛋白聚糖的传统生化分析。
