Camplejohn K L, Allard S A
Kennedy Institute of Rheumatology, London, UK.
Histochemistry. 1988;89(2):185-8. doi: 10.1007/BF00489922.
The intensity of safranin 'O' staining is directly proportional to the proteoglycan content in normal cartilage. Safranin 'O' has thus been used to demonstrate any changes that occur in articular disease. In this study, staining patterns obtained using monoclonal antibodies against the major components of cartilage proteoglycan chondroitin sulphate (anti CS) and keratan sulphate (anti KS), have been compared with those obtained with safranin 'O' staining, in both normal and arthritic tissues. In cartilage where safranin 'O' staining was not detectable, the monoclonal antibodies revealed the presence of both keratan and chondroitin sulphate. Thus, safranin 'O' is not a sensitive indicator of proteoglycan content in diseases where glycosaminoglaycan loss from cartilage has been severe.
番红O染色的强度与正常软骨中的蛋白聚糖含量成正比。因此，番红O已被用于证明关节疾病中发生的任何变化。在本研究中，将使用针对软骨蛋白聚糖硫酸软骨素（抗CS）和硫酸角质素（抗KS）主要成分的单克隆抗体获得的染色模式，与在正常组织和关节炎组织中用番红O染色获得的模式进行了比较。在无法检测到番红O染色的软骨中，单克隆抗体显示同时存在角质素和硫酸软骨素。因此，在软骨中糖胺聚糖损失严重的疾病中，番红O不是蛋白聚糖含量的敏感指标。
