Sanchez-Rivas C
Mol Gen Genet. 1982;185(2):329-33. doi: 10.1007/BF00330807.
A minimal medium containing horse serum is described on which Bacillus subtilis protoplasts revert to bacillary forms at high frequency (ca. 30%). Used as a plating medium for a mixture of polyethyleneglycol-treated protoplasts from two complementary polyauxotrophic parental strains, it selects the prototrophic fusion products efficiently, and also allows isolation of various auxotrophic recombinants. These prototrophs and recombinants amount respectively to 1% and 10% of the regenerated bacteria. We confirm that two types of prototrophs can be isolated after fusion: stable recombinants and complementing diploids, the latter segregating into various types of recombinants. Based on easily recognized colonial aspects, an approximate estimation of the proportion of the two types becomes possible when a spoOA mutation has been introduced in one of the parents. At least 50% of the prototrophic fusion products are complementing diploids. Incidently, the data also settle a controversy by showing the dominance of spoOA mutations in heterozygotic bacteria.
本文描述了一种含有马血清的基本培养基，枯草芽孢杆菌原生质体在该培养基上能高频（约30%）回复为杆菌形态。将其用作两种互补多营养缺陷型亲本菌株经聚乙二醇处理后的原生质体混合物的平板培养基时，它能高效筛选出原养型融合产物，还能分离出各种营养缺陷型重组体。这些原养型和重组体分别占再生细菌的1%和10%。我们证实融合后可分离出两种类型的原养型：稳定重组体和互补二倍体，后者会分离成各种类型的重组体。基于易于识别的菌落特征，当在其中一个亲本中引入spoOA突变时，就有可能大致估算出这两种类型的比例。至少50%的原养型融合产物是互补二倍体。顺便提一下，这些数据还通过显示spoOA突变在杂合细菌中的显性作用解决了一个争议。
