巨大芽孢杆菌原生质体的融合
Fusion of protoplasts of Bacillus megaterium.
作者信息
Fodor K, Alföldi L
出版信息
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2147-50. doi: 10.1073/pnas.73.6.2147.
Abstract
Protoplasts of two doubly auxotrophis strains of Bacillus megaterium were fused by nascent calcium phosphate or by polyethylene glycol treatment, and then allowed to revert to bacillary form on selection media. The colonies formed were analyzed and found to be mixed populations of individual bacteria apparently containing parental, recombinant, and segregating genotypes. The evidence suggested that segregation was transitory, lasting only for a few clonings and reisolations.
摘要
巨大芽孢杆菌的两个双重营养缺陷型菌株的原生质体通过新生磷酸钙或聚乙二醇处理进行融合，然后在选择培养基上使其回复为杆菌形态。对形成的菌落进行分析，发现它们是单个细菌的混合群体，显然包含亲本、重组和分离基因型。证据表明分离是短暂的，仅持续少数几次克隆和重新分离。
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