Loren A B, Matsuo Y, Charman D, Yokoyama M M
Transfusion. 1982 May-Jun;22(3):194-6. doi: 10.1046/j.1537-2995.1982.22382224939.x.
By sensitizing human red blood cells with antisera specific for corresponding blood group antigens and subsequent rosette formation, we were able to distinguish homozygosity of the antigens from heterozygosity. Three types of rosette assays were utilized: a protein A rosette, an immunoglobulin-coated bead rosette, and an erythrocyte-antibody rosette with human lymphocytes. In the Rh blood group system, we were able to demonstrate the dosage effect of the Rho(D) antigen by rosette assay in addition to determining that the deletion of the C and/or E alleles increased the rosette forming cell count when red blood cells with different genotypes were sensitized with Rh immune globulin (anti-D). The assay proved to be reproducible in distinguishing between homozygous and heterozygous Rh red blood cell antigens, and may be adaptable to study many antigenic markers on cell surfaces.
通过用针对相应血型抗原的抗血清使人类红细胞致敏并随后形成玫瑰花结，我们能够区分抗原的纯合性和杂合性。使用了三种类型的玫瑰花结试验：蛋白A玫瑰花结、免疫球蛋白包被珠玫瑰花结和人淋巴细胞红细胞抗体玫瑰花结。在Rh血型系统中，除了确定当用Rh免疫球蛋白（抗-D）使不同基因型的红细胞致敏时，C和/或E等位基因的缺失会增加玫瑰花结形成细胞计数外，我们还能够通过玫瑰花结试验证明Rho(D)抗原的剂量效应。该试验在区分纯合和杂合Rh红细胞抗原方面被证明是可重复的，并且可能适用于研究细胞表面的许多抗原标志物。
