Wooley R E, Gilbert J P, Shotts E B
Am J Vet Res. 1982 Jan;43(1):130-3.
Antibacterial effects against Proteus mirabilis, Salmonella typhimurium, and Aeromonas hydrophila were obtained with subminimal inhibitory concentrations of oxytetracycline and EDTA-tromethamine. Antibacterial effects were not observed with subminimal inhibitory concentrations of dimethyl sulfoxide plus oxytetracycline or with dimethyl sulfoxide plus EDTA-tromethamine. Using a 2-dimensional Microtiter checkerboard technique, inhibitory activities of the various combinations of solutions were studied, and isobolograms were plotted. A synergistic effect was seen with combinations of oxytetracycline and EDTA-tromethamine. The greatest synergistic effect was observed when the mixture was caused to react with P mirabilis. These findings were confirmed by kinetic studies of microbial death, using one-fourth minimal inhibitory concentrations of these preparations.
土霉素和乙二胺四乙酸- tromethamine的亚最小抑菌浓度对奇异变形杆菌、鼠伤寒沙门氏菌和嗜水气单胞菌有抗菌作用。亚最小抑菌浓度的二甲亚砜加土霉素或二甲亚砜加乙二胺四乙酸- tromethamine未观察到抗菌作用。使用二维微量滴定棋盘技术，研究了各种溶液组合的抑制活性，并绘制了等效线图。土霉素和乙二胺四乙酸- tromethamine组合具有协同作用。当混合物与奇异变形杆菌反应时，观察到最大的协同作用。使用这些制剂的四分之一最小抑菌浓度进行微生物死亡动力学研究证实了这些发现。
