Wooley R E, Jones M S, Gilbert J P, Shotts E B
Am J Vet Res. 1983 Jun;44(6):1154-8.
Combinations of EDTA-tromethamine and 7 antimicrobial agents [chloramphenicol, nalidixic acid, oxytetracycline, penicillin, polymyxin B, streptomycin, and triple sulfa (sulfamethazine, sulfapyridine, and sulfathiazole]) were tested for synergistic activities against Escherichia coli. Three in vitro tests were used including minimal inhibitory concentrations of the drugs, a 2-dimensional microtiter checkerboard technique, and bacterial inhibition studies. A strong synergistic inhibitory action was observed with combinations of EDTA-tromethamine plus penicillin and EDTA-tromethamine plus oxytetracycline. Combinations of EDTA-tromethamine plus chloramphenicol also had a synergistic action, but to a lesser extent than that with penicillin and oxytetracycline. When streptomycin or nalidixic acid was mixed with EDTA-tromethamine, a slight synergistic action was noticed. There was no synergistic effect recorded with combinations of EDTA-tromethamine and triple sulfa. Combinations of EDTA-tromethamine and polymyxin B had an antagonistic effect.
测试了乙二胺四乙酸-三羟甲基氨基甲烷与7种抗菌剂（氯霉素、萘啶酸、土霉素、青霉素、多粘菌素B、链霉素和三磺合剂[磺胺二甲嘧啶、磺胺吡啶和磺胺噻唑]）的组合对大肠杆菌的协同活性。使用了三种体外试验，包括药物的最低抑菌浓度、二维微量滴定棋盘法和细菌抑制研究。观察到乙二胺四乙酸-三羟甲基氨基甲烷加青霉素以及乙二胺四乙酸-三羟甲基氨基甲烷加土霉素的组合具有强烈的协同抑制作用。乙二胺四乙酸-三羟甲基氨基甲烷加氯霉素的组合也有协同作用，但程度小于与青霉素和土霉素的组合。当链霉素或萘啶酸与乙二胺四乙酸-三羟甲基氨基甲烷混合时，观察到轻微的协同作用。乙二胺四乙酸-三羟甲基氨基甲烷与三磺合剂的组合未记录到协同作用。乙二胺四乙酸-三羟甲基氨基甲烷和多粘菌素B的组合具有拮抗作用。
