Diamond A G, Triffitt J T, Herring G M
Arch Oral Biol. 1982;27(4):337-45. doi: 10.1016/0003-9969(82)90164-9.
Rabbit cortical bone powder was extracted with EDTA solutions at neutral pH and the soluble constituents fractionated by DEAE-cellulose chromatography. The acidic fractions obtained were further investigated by gel chromatography and chemical analysis. Two classes of proteoglycans were present; one containing chondroitin sulphate and the other containing material resembling keratan sulphate mixed with a smaller amount of chondroitin sulphate. Sialoglycoproteins were detected by specific chemical introduction of tritium label into the sialic acid residues of the acidic glycoprotein fraction. Following sodium dodecyl sulphate-polyacrylamide gel electrophoresis and fluorography, the presence of numerous distinct sialoglycoprotein components was demonstrated.
兔皮质骨粉用中性pH值的乙二胺四乙酸（EDTA）溶液提取，其可溶性成分通过二乙氨基乙基纤维素（DEAE-纤维素）色谱法进行分离。对得到的酸性组分进一步采用凝胶色谱法和化学分析法进行研究。存在两类蛋白聚糖；一类含有硫酸软骨素，另一类含有类似于硫酸角质素的物质，并混有少量硫酸软骨素。通过将氚标记特异性化学引入酸性糖蛋白组分的唾液酸残基中来检测唾液酸糖蛋白。经过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和放射自显影后，证实存在许多不同的唾液酸糖蛋白组分。
