Garry V F, Nesbitt M, White J, Vosika G
In Vitro. 1978 Jun;14(6):495-501. doi: 10.1007/BF02616089.
A bone-marrow culture system is described that provides a simple, quantitative and rapid assessment of marrow bone cells in vitro. Aggregation of bone-marrow cells, an in vitro phenomenon, occurs within 24 hr of culture and is observed utilizing Millipore filters. Daily quantitation shows both an increase in the number and a change in the morphology of these aggregates. The maximum number of aggregates is achieved on the 2nd or 3rd day of incubation. Histologically, aggregates are composed of myeloid, mononuclear and mesenchymal fibroblastic cells. Mesenchymal cells form a matrix for apposed mononuclear and myeloid cells. Scanning electron micrographs show intimate cell contact and spreading by the marrow cells. Fluctuation of the absolute numbers of various cell types are observed. The system can be utilized for long-term culture of bone marrow.
本文描述了一种骨髓培养系统，该系统可在体外对骨髓骨细胞进行简单、定量且快速的评估。骨髓细胞聚集是一种体外现象，在培养24小时内发生，可利用密理博滤膜进行观察。每日定量分析显示这些聚集体的数量增加且形态发生变化。在培养的第2天或第3天达到聚集体的最大数量。组织学上，聚集体由髓系、单核和间充质成纤维细胞组成。间充质细胞为相邻的单核和髓系细胞形成基质。扫描电子显微镜照片显示骨髓细胞之间有紧密的细胞接触和铺展。观察到各种细胞类型的绝对数量有波动。该系统可用于骨髓的长期培养。
