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一种新型的枯草芽孢杆菌松弛突变体。

A new relaxed mutant of Bacillus subtilis.

作者信息

Price V L, Gallant J A

出版信息

J Bacteriol. 1982 Feb;149(2):635-41. doi: 10.1128/jb.149.2.635-641.1982.

DOI:10.1128/jb.149.2.635-641.1982
PMID:6173376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216552/
Abstract

A new relaxed mutant of Bacillus subtilis was isolated by screening Rifr clones for alterations in stringent control. The Rifr relaxed mutant which is described was found to contain a second-site mutation conferring a relaxed response to an energy source downshift and was partially relaxed after amino acid starvation. The new rel locus, called relG, was distinct from the two other known rel loci in B. subtilis, relA, and relC.

摘要

通过筛选利福平抗性(Rifr)克隆以寻找严紧控制方面的改变,分离出了一种新型的枯草芽孢杆菌松弛突变体。所描述的这种Rifr松弛突变体被发现含有一个第二位点突变,该突变赋予了对能量源下调的松弛反应,并且在氨基酸饥饿后部分松弛。这个新的rel位点被称为relG,它与枯草芽孢杆菌中另外两个已知的rel位点relA和relC不同。

相似文献

1
A new relaxed mutant of Bacillus subtilis.一种新型的枯草芽孢杆菌松弛突变体。
J Bacteriol. 1982 Feb;149(2):635-41. doi: 10.1128/jb.149.2.635-641.1982.
2
Degradation of aspartate transcarbamylase in Bacillus subtilis is deficient in rel mutants but is not mediated by guanosine polyphosphates.枯草芽孢杆菌中天冬氨酸转氨甲酰酶的降解在rel突变体中存在缺陷,但不是由鸟苷多聚磷酸介导的。
J Bacteriol. 1984 May;158(2):746-8. doi: 10.1128/jb.158.2.746-748.1984.
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The stringent response to unacylated tRNA, energy-and temperature-downshift in Bacillus stearothermophilus.嗜热脂肪芽孢杆菌对未酰化tRNA、能量和温度下降的严格反应。
Arch Microbiol. 1981 Mar;129(1):29-31. doi: 10.1007/BF00417174.
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Eur J Biochem. 1978 Apr 17;85(2):517-28. doi: 10.1111/j.1432-1033.1978.tb12267.x.
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Evidence that Bacillus subtilis sporulation induced by the stringent response is caused by the decrease in GTP or GDP.有证据表明,由严紧反应诱导的枯草芽孢杆菌孢子形成是由GTP或GDP的减少引起的。
J Bacteriol. 1982 Aug;151(2):1062-5. doi: 10.1128/jb.151.2.1062-1065.1982.
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Induction of stringent response by streptomycin in Bacillus subtilis cells.链霉素在枯草芽孢杆菌细胞中诱导严谨反应。
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Thiostrepton-resistant mutants exhibit relaxed synthesis of RNA.硫链丝菌素抗性突变体表现出RNA合成的松弛现象。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5993-7. doi: 10.1073/pnas.75.12.5993.
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Nitrofurantoin prompts the stringent response in Bacillus subtilis.呋喃妥因可引发枯草芽孢杆菌的严谨反应。
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An RNA-DNA copolymer whose synthesis is correlated with the transcriptional requirement for chromosomal initiation in Bacillus subtilis contains ribosomal RNA sequences.一种 RNA-DNA 共聚物,其合成与枯草芽孢杆菌染色体起始的转录需求相关,包含核糖体 RNA 序列。
Proc Natl Acad Sci U S A. 1985 Jun;82(11):3586-90. doi: 10.1073/pnas.82.11.3586.
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Helicobacter pylori: a eubacterium lacking the stringent response.幽门螺杆菌:一种缺乏严谨反应的真细菌。
J Bacteriol. 1999 Jan;181(2):552-5. doi: 10.1128/JB.181.2.552-555.1999.
4
Bacillus subtilis relG mutant: defect in glucose uptake.枯草芽孢杆菌relG突变体:葡萄糖摄取缺陷。
J Bacteriol. 1983 Jan;153(1):270-3. doi: 10.1128/jb.153.1.270-273.1983.
5
Revised genetic linkage map of Bacillus subtilis.枯草芽孢杆菌的修订遗传连锁图谱。
Microbiol Rev. 1985 Jun;49(2):158-79. doi: 10.1128/mr.49.2.158-179.1985.

本文引用的文献

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Transcriptional inhibition and production of guanosine polyphosphates in Bacillus subtilis.枯草芽孢杆菌中的转录抑制和鸟苷多磷酸的产生
J Bacteriol. 1981 Sep;147(3):752-6. doi: 10.1128/jb.147.3.752-756.1981.
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Genetics and physiology of the rel system of Bacillus subtilis.枯草芽孢杆菌rel系统的遗传学与生理学
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Isolation and characterization of an RNA relaxed mutant of B. subtilis.枯草芽孢杆菌RNA松弛突变体的分离与鉴定
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Proc Natl Acad Sci U S A. 1975 Nov;72(11):4389-93. doi: 10.1073/pnas.72.11.4389.
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A rapid and accurate procedure for assaying DNA polymerase, RNA polymerase, or ribosome dependent protein synthesis.
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A rapid test for the rel A mutation in E. coli.一种用于检测大肠杆菌中rel A突变的快速检测方法。
Biochem Biophys Res Commun. 1976 Apr 5;69(3):751-8. doi: 10.1016/0006-291x(76)90939-6.
8
Reconstitution studies show that rifampicin resistance is determined by the largest polypeptide of Bacillus subtilis RNA polymerase.重组研究表明,利福平耐药性由枯草芽孢杆菌RNA聚合酶的最大多肽决定。
J Biol Chem. 1977 Dec 25;252(24):9024-31.
9
Escherichia coli mutant containing a large deletion from relA to argA.含有从relA到argA的大片段缺失的大肠杆菌突变体。
J Bacteriol. 1979 May;138(2):530-4. doi: 10.1128/jb.138.2.530-534.1979.
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Nonsense and insertion mutants in the relA gene of E. coli: cloning relA.
Cell. 1978 Dec;15(4):1187-97. doi: 10.1016/0092-8674(78)90045-4.