Yokota K, Kito M
J Bacteriol. 1982 Aug;151(2):952-61. doi: 10.1128/jb.151.2.952-961.1982.
Phosphatidylglycerol was pulse-labeled with radioactive lipid precursors in a serine auxotroph of Escherichia coli. Most of the radioactivity of phosphatidylglycerol labeled in a serine-depleted medium was transferred to phosphatidylethanolamine during a chase in the presence of L-serine, but not in its absence. Metabolism of fatty acyl moieties labeled with [1-14C]acetate, acylated glycerol moieties labeled with [2-3H]glycerol, and phosphate moieties labeled with 32Pi, followed by a chase in the presence of cerulenin, showed that the intact phosphatidyl moiety of phosphatidylglycerol was transferred to phosphatidylethanolamine. The composition of phosphatidylethanolamine molecular species was unaltered and not perturbed by the transfer of the phosphatidyl moiety of phosphatidylglycerol. The increase of phosphatidylethanolamine with a concomitant decrease of phosphatidylglycerol was not coupled with the postulated turnover of phosphatidylglycerol to membrane-derived oligosaccharides and lipoprotein. It is suggested that phosphatidylglycerol is capable of providing its phosphatidyl moiety for the production of phosphatidylethanolamine in response to the relief of serine limitation by addition of L-serine.
在大肠杆菌的丝氨酸营养缺陷型菌株中，用放射性脂质前体对磷脂酰甘油进行脉冲标记。在丝氨酸耗尽的培养基中标记的磷脂酰甘油的大部分放射性，在L-丝氨酸存在的追踪过程中转移到了磷脂酰乙醇胺，但在其不存在时则没有。用[1-14C]乙酸盐标记的脂肪酰部分、用[2-3H]甘油标记的酰化甘油部分和用32Pi标记的磷酸部分的代谢，随后在浅蓝菌素存在下进行追踪，结果表明磷脂酰甘油完整的磷脂部分转移到了磷脂酰乙醇胺。磷脂酰乙醇胺分子种类的组成没有改变，也没有因磷脂酰甘油磷脂部分的转移而受到干扰。磷脂酰乙醇胺增加而磷脂酰甘油相应减少，这与推测的磷脂酰甘油向膜衍生寡糖和脂蛋白的周转无关。有人提出，磷脂酰甘油能够在添加L-丝氨酸缓解丝氨酸限制时，为磷脂酰乙醇胺的产生提供其磷脂部分。
