通过磷脂囊泡融合将磷脂酰甘油掺入鼠伤寒沙门氏菌完整细胞的胞壁质脂蛋白中。
Incorporation of phosphatidylglycerol into murein lipoprotein in intact cells of Salmonella typhimurium by phospholipid vesicle fusion.
作者信息
Chattopadhyay P K, Lai J S, Wu H C
出版信息
J Bacteriol. 1979 Jan;137(1):309-12. doi: 10.1128/jb.137.1.309-312.1979.
Abstract
The biosynthesis of the diglyceride moiety of murein lipoprotein was studied by fusion of labeled phospholipid vesicles with intact cells of Salmonella typhimurium. Phosphatidylglycerol was found to be an excellent donor for the glyceryl moiety in lipoprotein, whereas phosphatidylethanolamine and cardiolipin were not. The incorporation of radioactivity from monoacyl-phosphatidylglycerol into lipoprotein can be attributed to its conversion to phosphatidylglycerol. The results strongly support our hypothesis that the glyceryl residue covalently linked to murein lipoprotein is derived from the nonacylated glycerol moiety of phosphatidylglycerol.
摘要
通过将标记的磷脂囊泡与鼠伤寒沙门氏菌的完整细胞融合，研究了胞壁酰脂蛋白甘油二酯部分的生物合成。发现磷脂酰甘油是脂蛋白中甘油部分的优良供体，而磷脂酰乙醇胺和心磷脂则不是。单酰磷脂酰甘油中的放射性掺入脂蛋白可归因于其转化为磷脂酰甘油。结果有力地支持了我们的假设，即与胞壁酰脂蛋白共价连接的甘油残基源自磷脂酰甘油的未酰化甘油部分。
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