通过磷脂囊泡融合将磷脂的酰基部分掺入大肠杆菌完整细胞的胞壁质脂蛋白中。
Incorporation of acyl moieties of phospholipids into murein lipoprotein in intact cells of Escherichia coli by phospholipid vesicle fusion.
作者信息
Lai J S, Wu H C
出版信息
J Bacteriol. 1980 Oct;144(1):451-3. doi: 10.1128/jb.144.1.451-453.1980.
Abstract
The biosynthesis of the acyl moieties in murein lipoprotein was studied by fusion of [3H]palmitate-labeled phospholipid vesicles with intact cells of an fadD mutant of Escherichia coli. A linear increase in the incorporation of [3H]palmitate radioactivity into both the ester- and amide-linked fatty acids in lipoprotein was observed during a 3-h chase after the fusion. Addition of chloramphenicol completely prevented the incorporation of [3H]palmitate from phospholipids to lipoprotein. These results strongly support our hypothesis that the acyl moieties in phospholipids are the precursors for the fatty acids in murein lipoprotein of E. coli. Among the major glycerophosphatides in E. coli, no specificity was observed regarding the efficacy of the donor.
摘要
通过将[3H]棕榈酸标记的磷脂囊泡与大肠杆菌fadD突变体的完整细胞融合，研究了胞壁质脂蛋白中酰基部分的生物合成。融合后3小时的追踪过程中，观察到脂蛋白中[3H]棕榈酸放射性掺入酯键连接和酰胺键连接的脂肪酸均呈线性增加。添加氯霉素完全阻止了[3H]棕榈酸从磷脂掺入脂蛋白。这些结果有力地支持了我们的假设，即磷脂中的酰基部分是大肠杆菌胞壁质脂蛋白中脂肪酸的前体。在大肠杆菌的主要甘油磷脂中，未观察到供体效力的特异性。
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