Clarke S H, Claflin J L, Rudikoff S
Proc Natl Acad Sci U S A. 1982 May;79(10):3280-4. doi: 10.1073/pnas.79.10.3280.
Complete heavy (H) chain variable region (V region; amino acids 1-118) sequences have been determined for three phosphocholine (PCho)-binding monoclonal antibodies of CBA mouse strain origin. Two of these were found to differ from the sequence of the BALB/c T15 germline VH segment (segment of the V region that includes amino acids 1-95) at four positions but were identical to the allelic form of T15 (C3) found in C57BL. The third VH segment, HP101.6G6 (6G6), was clearly the product of a second, related VH gene, probably the allele of the BALB/c V11 gene, a second member of the P-Cho VH gene family. Thus, more than one VH gene is capable of encoding heavy chains of PCho-binding antibodies. The 6G6 VH segment differs from VII at seven positions; four of these distinguishing amino acids are encoded in other membranes of the PCho VH gene family. We postulate that the origin of the 6G6 VH sequence can most easily be explained by a process of gene conversion occurring between the least three members of the PCho VH family.
已确定源自CBA小鼠品系的三种结合磷酸胆碱（PCho）的单克隆抗体的完整重链（H）可变区（V区；氨基酸1 - 118）序列。发现其中两种在四个位置上与BALB/c T15种系VH区段（V区中包含氨基酸1 - 95的区段）的序列不同，但与在C57BL中发现的T15（C3）等位基因形式相同。第三个VH区段HP101.6G6（6G6）显然是第二个相关VH基因的产物，可能是BALB/c V11基因的等位基因，P-Cho VH基因家族的第二个成员。因此，不止一个VH基因能够编码结合PCho抗体的重链。6G6 VH区段在七个位置上与VII不同；这些区分性氨基酸中的四个在PCho VH基因家族的其他成员中编码。我们推测，6G6 VH序列的起源最容易通过PCho VH家族至少三个成员之间发生的基因转换过程来解释。
