Igarashi K, Kakegawa T, Hirose S
Biochim Biophys Acta. 1982 May 31;697(2):185-92. doi: 10.1016/0167-4781(82)90076-8.
An explanation for the fragility of 30 S ribosomal subunits of Bacillus subtilis has been studied. Degradation of 16 S ribosomal RNA, rather than degradation of ribosomal proteins, was found to cause the inactivation of 30 S subunits. Although RNAases were bound specifically to 30 S ribosomal subunits, the RNAases were able to function. Spermidine was found to contribute to the stabilization of 30 S ribosomal subunits by inhibiting the degradation of 16 S ribosomal RNA. A high concentration of Mg2+ also stabilized the 30 S ribosomal subunits of Bacillus subtilis. The polypeptide synthetic activity of 30 S ribosomal subunits prepared in the presence of spermidine was at least 4-times greater than that of 30 S ribosomal subunits prepared in the absence of spermidine; this activity was maintained without any loss for 3 months at -70 degrees C.
对枯草芽孢杆菌30 S核糖体亚基的脆弱性进行了研究。发现16 S核糖体RNA的降解而非核糖体蛋白的降解导致30 S亚基失活。尽管核糖核酸酶特异性结合到30 S核糖体亚基上，但这些核糖核酸酶仍能发挥作用。发现亚精胺通过抑制16 S核糖体RNA的降解有助于30 S核糖体亚基的稳定。高浓度的Mg2+也能稳定枯草芽孢杆菌的30 S核糖体亚基。在亚精胺存在下制备的30 S核糖体亚基的多肽合成活性至少比在无亚精胺情况下制备的30 S核糖体亚基高4倍；该活性在-70℃下可保持3个月无任何损失。
