在多不饱和脂肪酸存在的情况下生长对组织培养神经元细胞动作电位的改变。
Alteration of the action potential of tissue cultured neuronal cells by growth in the presence of a polyunsaturated fatty acid.
作者信息
Saum W R, McGee R, Love J
出版信息
Cell Mol Neurobiol. 1981 Sep;1(3):319-24. doi: 10.1007/BF00710687.
The effects of alterations in membrane phospholipid fatty acid composition on the excitability of neuroblastoma X glioma hybrid cells, clone NG108-15, were examined using intracellular recording techniques. Cells were grown in the presence of arachidonate (20:4) added to the culture medium as a complex with bovine serum albumin. Exposure of the cells to 20:4 for 3-21 days produced a 40% decrease in the maximum rate of rise of the action potential (dV/dt) with a small change in its amplitude. The resting membrane potential and passive properties of the cells were unaffected. An effect of 20:4 was not observed until 24 hr after treatment and increased over the next 2 days. The phospholipid content of 20:4 and its metabolite 22:4 increased from 6.9% to 25.3% of total fatty acids during approximately the same time span. It is concluded that the action potential dV/dt can be altered by changes in membrane lipid composition.
采用细胞内记录技术，研究了膜磷脂脂肪酸组成的改变对神经母细胞瘤X胶质瘤杂交细胞系克隆NG108 - 15兴奋性的影响。细胞在添加花生四烯酸（20:4）的培养基中生长，花生四烯酸与牛血清白蛋白形成复合物。将细胞暴露于20:4中3至21天，动作电位的最大上升速率（dV/dt）降低了40%，而其幅度变化较小。细胞的静息膜电位和被动特性未受影响。直到处理后24小时才观察到20:4的作用，且在接下来的2天内作用增强。在大约相同的时间跨度内，20:4及其代谢产物22:4的磷脂含量从总脂肪酸的6.9%增加到25.3%。得出结论，动作电位dV/dt可因膜脂质组成的变化而改变。
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