McGee R
Biochim Biophys Acta. 1981 Jan 26;663(1):314-28. doi: 10.1016/0005-2760(81)90217-4.
As a first step in studying the effects of membrane lipid modification on complex cellular functions we have modified the membrane fatty acid composition of the neuroblastoma X glioma hybrid clone, NG108-15. These cultured cells were chosen because they exhibit many complex neuronal functions in vitro. Unsaturated fatty acids (oleate, linoleate, linolenate and arachidonate) were accumulated, metabolized and esterified by the cells. These unsaturated fatty acids stimulated cell growth, whereas saturated fatty acids were toxic to the cells. Changes as large as 40-fold in the ratio of monounsaturated/polyunsaturated fatty acids in the membrane phospholipids were produced by addition of fatty acids directly to serum-containing culture medium. As a result of the exposure of NG108-15 cells to unsaturated fatty acids the amount of phosphatidylethanolamine in the cells was increased by as much as 60%. Polyunsaturated fatty acids also caused a small decrease in the membrane cholesterol/phospholipid molar ratio. These experiments demonstrate that large changes in membrane fatty acid composition can be created in clonal cells capable of differentiated neuronal activities. Additional changes in membrane lipid composition also appear to be induced by these manipulations. The question of the importance of specific membrane lipid composition to neuronal cellular function now can be addressed.
作为研究膜脂修饰对复杂细胞功能影响的第一步，我们对神经母细胞瘤X胶质瘤杂交克隆NG108 - 15的膜脂肪酸组成进行了修饰。选择这些培养细胞是因为它们在体外表现出许多复杂的神经元功能。不饱和脂肪酸（油酸、亚油酸、亚麻酸和花生四烯酸）被细胞积累、代谢和酯化。这些不饱和脂肪酸刺激细胞生长，而饱和脂肪酸对细胞有毒。通过将脂肪酸直接添加到含血清的培养基中，膜磷脂中单不饱和/多不饱和脂肪酸的比例变化高达40倍。由于NG108 - 15细胞暴露于不饱和脂肪酸，细胞中磷脂酰乙醇胺的量增加了多达60%。多不饱和脂肪酸还导致膜胆固醇/磷脂摩尔比略有下降。这些实验表明，在能够进行分化神经元活动的克隆细胞中，可以产生膜脂肪酸组成的巨大变化。这些操作似乎还诱导了膜脂组成的其他变化。现在可以探讨特定膜脂组成对神经元细胞功能的重要性问题。
