用于检测淋病奈瑟菌抗体的放射免疫测定法。
Radioimmunoassay for detection of antibodies to Neisseria gonorrhoeae.
作者信息
Usategui M, Savard E V, Mondabaugh S M, Keigher N L
出版信息
J Clin Microbiol. 1982 Jun;15(6):1001-8. doi: 10.1128/jcm.15.6.1001-1008.1982.
A radioimmunoassay has been developed and evaluated for the serological diagnosis of gonorrhea. Purified gonococcal antigen was obtained from a culture of Neisseria gonorrhoeae (B370) and labeled with 125I for use in a double-antibody test system. The test was evaluated in populations segregated by sex and risk. The specificity of the assay in females was 90.2% (55/61) in low risk, 82.2% (2,245/ 2,732) in medium risk, and 54.1% (335/619) in high risk. The sensitivity was 69% (20/29) in medium risk and 78.3% (288/367) in high risk. In males, test specificity was 92.3% (24/26) in low risk and 50% (48/96) in high risk. The sensitivity was 70.8% (143/202) in the high-risk group. The data in this study indicate that this assay should not be employed for screening of either high- or medium-risk populations.
已开发并评估了一种用于淋病血清学诊断的放射免疫测定法。从淋病奈瑟菌（B370）培养物中获得纯化的淋球菌抗原，并用125I进行标记，用于双抗体检测系统。该检测在按性别和风险分层的人群中进行了评估。该检测在低风险女性中的特异性为90.2%（55/61），中风险女性中为82.2%（2245/2732），高风险女性中为54.1%（335/619）。中风险女性的敏感性为69%（20/29），高风险女性中为78.3%（288/367）。在男性中，低风险男性的检测特异性为92.3%（24/26），高风险男性中为50%（48/96）。高风险男性组的敏感性为70.8%（143/202）。本研究中的数据表明，该检测不应用于高风险或中风险人群的筛查。
Zotero 插件