使用全细胞的酶联免疫吸附测定（ELISA）检测淋病抗体。
Enzyme-linked immunosorbent assay (ELISA) to detect antibodies in gonorrhea using whole cells.
作者信息
Ison C A, Hadfield S G, Glynn A A
出版信息
J Clin Pathol. 1981 Sep;34(9):1040-3. doi: 10.1136/jcp.34.9.1040.
Abstract
The choice of an antigen that will adequately differentiate between infected and non-infected patients has been a problem in detecting gonococcal antibodies for diagnosis. We have used the sensitive technique of ELISA to test various serotypes of Neisseria gonorrhoeae for their suitability as antigens. Whole cells of each serotype were attached to polystyrene plates using poly-L-lysine, N gonorrhoeae, strain H1 type 1 was used to detect antibodies in patients with known clinical history and then as a standard to evaluate the ability of different serotypes to differentiate between infected and non-infected groups.
摘要
在检测用于诊断的淋球菌抗体时，选择一种能够充分区分感染患者和未感染患者的抗原一直是个问题。我们采用了灵敏的酶联免疫吸附测定（ELISA）技术，来检测淋病奈瑟菌的各种血清型作为抗原的适用性。使用聚-L-赖氨酸将每种血清型的全细胞附着于聚苯乙烯板上，用淋病奈瑟菌H1型1菌株检测有已知临床病史患者的抗体，然后以此作为标准来评估不同血清型区分感染组和未感染组的能力。
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本文引用的文献
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Quantitative determination of antibody to gonococcal pili. Changes in antibody levels with gonococcal infection.淋菌菌毛抗体的定量测定。淋菌感染时抗体水平的变化。
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