抗乙酰胆碱受体抗体：在轻链和亚类测定中使用聚乙二醇辅助沉淀抗体-受体复合物。
Anti-acetylcholine receptor antibody: use of polyethylene glycol as an aid to precipitation of antibody-receptor complexes in determination of light chain and subclass.
作者信息
Vincent A, Bilkhu M
出版信息
J Immunol Methods. 1982;51(3):359-69. doi: 10.1016/0022-1759(82)90404-5.
PMID:6809835
Abstract
Polyethylene glycol 6000 (PEG) was used as an aid to precipitation of antibody-acetylcholine receptor (AChR) complexes. In the absence of anti-human IgG, 8% PEG can be used to precipitate antibody-AChR complexes. In the presence of low titre specific antiserum, 3% PEG selectively precipitates anti-IgG-IgG-AChR complexes and allows analysis of light chain and subclass contribution to the anti-AChR. The specificity of the various antisera is presented and results in 16 patients described.
摘要
聚乙二醇6000（PEG）被用作辅助沉淀抗体-乙酰胆碱受体（AChR）复合物。在没有抗人IgG的情况下，8%的PEG可用于沉淀抗体-AChR复合物。在低滴度特异性抗血清存在的情况下，3%的PEG可选择性沉淀抗IgG-IgG-AChR复合物，并可分析轻链和亚类对抗AChR的贡献。文中介绍了各种抗血清的特异性，并描述了16例患者的结果。
Zotero 插件