Cammer W, Snyder D S, Zimmerman T R, Farooq M, Norton W T
J Neurochem. 1982 Feb;38(2):360-7. doi: 10.1111/j.1471-4159.1982.tb08637.x.
Glycerol phosphate dehydrogenase (GPDH), glucose-6-phosphate dehydrogenase (G6PDH), and lactate dehydrogenase (LDH) activities were determined in oligodendrocytes, neurons, and astrocytes isolated from the brains of developing rats. The activity of each enzyme was significantly lower in both neurons and astrocytes than in oligodendrocytes. The GPDH activity in oligodendrocytes increased more than 4-fold during development, and at 120 days cells of this type had 1.4-fold the specific activity of forebrain homogenates. The G6PDH activities in oligodendrocytes from 10-day-old rats were 1.4-fold the activities in the forebrain homogenates. The activities of this enzyme in oligodendrocytes were progressively lower at later ages, such that at 120 days the cells had 0.8 times the specific activities of homogenates. The oligodendrocytes had 0.6 times the homogenate activities of LDH at 10 days, and this ratio had decreased to 0.2 by 120 days. These enzymes were also measured in myelin isolated from 20-, 60-, and 120-day-old rats. By 120 days the specific activities of G6PDH and LDH in myelin were less than 8% of the respective activities in homogenates. The GPDH activity in myelin was, however, at least 20% the specific activity in the homogenates, even in the oldest animals. It is proposed that LDH could be used as a marker for oligodendroglial cytoplasm in subfractions of myelin and in myelin-related membrane vesicles.
测定了从发育中大鼠大脑分离出的少突胶质细胞、神经元和星形胶质细胞中的甘油磷酸脱氢酶（GPDH）、葡萄糖-6-磷酸脱氢酶（G6PDH）和乳酸脱氢酶（LDH）活性。神经元和星形胶质细胞中每种酶的活性均显著低于少突胶质细胞。少突胶质细胞中的GPDH活性在发育过程中增加了4倍多，在120天时，这类细胞的比活性是前脑匀浆的1.4倍。10日龄大鼠少突胶质细胞中的G6PDH活性是前脑匀浆活性的1.4倍。该酶在少突胶质细胞中的活性在后期逐渐降低，以至于在120天时，细胞的比活性是匀浆的0.8倍。少突胶质细胞在10天时的LDH活性是匀浆活性的0.6倍，到120天时，这一比例降至0.2。还测定了从20日龄、60日龄和120日龄大鼠分离出的髓磷脂中的这些酶。到120天时，髓磷脂中G6PDH和LDH的比活性分别低于匀浆中相应活性的8%。然而，即使在最年长的动物中，髓磷脂中的GPDH活性至少是匀浆中比活性的20%。有人提出，LDH可作为髓磷脂亚组分和髓磷脂相关膜泡中少突胶质细胞质的标志物。
