用传染性单核细胞增多症血清的保罗-邦内尔抗体进行凝胶中的双向扩散试验。
Double diffusion in gel tests with Paul-Bunnell antibodies of infectious mononucleosis sera.
作者信息
Milgrom F, Loza U, Kano K
出版信息
Int Arch Allergy Appl Immunol. 1975;48(1):82-93. doi: 10.1159/000231294.
Double diffusion tests in gel were employed for studies of reactions between infectious mononucleosis sera and extracts of bovine, sheep and equine erythrocyte stromata. The extracts were obtained by ultrasonication of stromata prepared from trypsin-digested erythrocytes. The reaction with bovine stroma extract was composed, in many instances, of two lines. A single line was observed in reactions with sheep and equine stroma extracts. This line merged into a reaction of partial or complete identy with one of the lines formed with bovine stroma extract. Evidence was obtained that some infectioous mononucleosis sera may contain heterophile (Paul-Bunnell) antibodies belonging to IgG class in addition to those of IgM nature.
采用凝胶双扩散试验研究传染性单核细胞增多症血清与牛、羊和马红细胞基质提取物之间的反应。提取物通过对用胰蛋白酶消化的红细胞制备的基质进行超声处理获得。在许多情况下，与牛基质提取物的反应由两条线组成。在与羊和马基质提取物的反应中观察到一条单线。这条线与用牛基质提取物形成的线之一合并为部分或完全相同的反应。有证据表明，一些传染性单核细胞增多症血清除了含有IgM性质的嗜异性（保罗 - 邦内尔）抗体外，可能还含有属于IgG类的嗜异性抗体。
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