Dietz J N, Cole B C
Infect Immun. 1982 Aug;37(2):811-9. doi: 10.1128/iai.37.2.811-819.1982.
Viable Mycoplasma arthritidis and supernatants from M. arthriditis cultures produced marked morphological changes in the J774.1 continuous macrophage line similar to those seen during activation of these cells by Mycobacterium bovis BCG cell walls. The mycoplasma-treated macrophages developed pronounced tumoricidal activity against syngenic 3T12-3 target cells and developed an enhanced capacity for the killing of intracellular listeriae, including both virulent and laboratory-maintained strains. Increased acid phosphatase levels and [14C]glucosamine uptake were also seen. We conclude that mycoplasmas can profoundly alter the functions of macrophages, an event which may not only have in vivo significance with regard to disease pathogenesis, but which may pose considerable problems for in vitro work when unsuspected mycoplasma contamination is present.
活的关节炎支原体及关节炎支原体培养物的上清液，在J774.1连续巨噬细胞系中引起了显著的形态学变化，类似于牛分枝杆菌卡介苗细胞壁激活这些细胞时所观察到的变化。经支原体处理的巨噬细胞对同基因3T12 - 3靶细胞产生了明显的杀瘤活性，并增强了杀灭细胞内李斯特菌的能力，包括强毒株和实验室保存菌株。还观察到酸性磷酸酶水平升高和[14C]葡萄糖胺摄取增加。我们得出结论，支原体可深刻改变巨噬细胞的功能，这一事件不仅在疾病发病机制方面可能具有体内意义，而且当存在未被怀疑的支原体污染时，可能给体外研究带来相当大的问题。
