阿非科林对经氯化钠处理的海拉细胞核中DNA合成的抑制模式。
The inhibitory mode of aphidicolin on DNA synthesis in NaCl-treated HeLa cell nuclei.
作者信息
Oguro M, Nagano H
出版信息
J Biochem. 1982 Jun;91(6):2091-4. doi: 10.1093/oxfordjournals.jbchem.a133903.
Isolated HeLa cell nuclei were treated with NaCl at various concentrations and inhibition by aphidicolin of DNA synthesis in the treated nuclei was studied. The inhibition was either noncompetitive or of the mixed type with respect to each dNTP when the nuclei were treated with NaCl at concentrations lower than 0.08 M. However, aphidicolin was a competitive inhibitor with respect to dCTP and a non-competitive or mixed type inhibitor with respect to the other 3 dNTPs when they were treated with NaCl at concentrations higher than 0.1 M. These results suggest the presence of nuclear factor(s) responsible for the changes in the inhibitory mode of aphidicolin on endogenous nuclear DNA synthesis.
用不同浓度的氯化钠处理分离的海拉细胞核，并研究经处理的细胞核中阿非科林对DNA合成的抑制作用。当用浓度低于0.08M的氯化钠处理细胞核时，阿非科林对每种脱氧核苷三磷酸（dNTP）的抑制作用要么是非竞争性的，要么是混合型的。然而，当用浓度高于0.1M的氯化钠处理细胞核时，阿非科林对dCTP是竞争性抑制剂，对其他三种dNTP是非竞争性或混合型抑制剂。这些结果表明存在负责阿非科林对内源性细胞核DNA合成抑制模式变化的核因子。
Zotero 插件