Sameshima H, Taya K, Sasamoto S, Etoh T
J Endocrinol. 1982 Sep;94(3):339-45. doi: 10.1677/joe.0.0940339.
Different numbers of rat pituitary glands, taken from male and female rats before and after puberty, were transplanted into various sites in female rats at different stages of the oestrous cycle. Ovulation was checked by counting ova in oviducts on the next expected day of ovulation. Ovulation was induced by transplantation beneath the kidney capsule in early dioestrus of half, one or three glands from 35- to 41-day-old male rats (18.6+/-3.1 (S.E.M.), 32.6+/-2.8 and 49.8+/-4.8 ova shed respectively). The transplantation of glands from mature female rats did not induce supraovulation but inhibited the expected ovulation. The most effective stage for inducing superovulation was early dioestrus and, to a lesser extent, most effective stage for inducing superovulation was early dioestrus and, to a lesser extent, oestrus; transplantation during late dioestrus was ineffective. The effective sites of transplantation were beneath the kidney capsule and intramuscularly but not subcutaneously. Representative pituitary glands from 35- to 41-day-old male rats and adult female rats were assayed for LH and FSH content to interpret the mechanisms of superovulation . The pituitary glands from the male rats contained larger amounts of LH and especially of FSH than those found in the female rats. The experiments indicated that superovulation can be induced successfully by the transplantation of a single pituitary gland from male and immature female rats without any additional treatment with human chorionic gonadotrophin; the failure of the female pituitary transplants to induce superovulation may be due to the insufficient content of LH and FSH.
取处于青春期前后的雄性和雌性大鼠的不同数量垂体，移植到处于发情周期不同阶段的雌性大鼠的不同部位。在下一个预期排卵日，通过计算输卵管中的卵子数量来检查排卵情况。将35至41日龄雄性大鼠的半个、一个或三个垂体移植到动情后期的雌性大鼠肾包膜下可诱导排卵（分别排出18.6±3.1（标准误）、32.6±2.8和49.8±4.8个卵子）。移植成熟雌性大鼠的垂体不能诱导超排卵，反而会抑制预期的排卵。诱导超排卵最有效的阶段是动情后期，在较小程度上，发情期也有效；动情间期后期移植无效。有效的移植部位是肾包膜下和肌肉内，而不是皮下。对35至41日龄雄性大鼠和成年雌性大鼠的代表性垂体进行促黄体生成素（LH）和促卵泡生成素（FSH）含量测定，以解释超排卵的机制。雄性大鼠的垂体中LH尤其是FSH的含量比雌性大鼠的垂体中含量高。实验表明，移植来自雄性和未成熟雌性大鼠的单个垂体，无需额外用人绒毛膜促性腺激素处理，即可成功诱导超排卵；雌性垂体移植未能诱导超排卵可能是由于LH和FSH含量不足。
